poster

MMM 2022

November 07, 2022

Minneapolis, United States

Portable Magnetoresistive Device for the Simultaneous Detection of Dengue RNA and Anti dengue Antibodies

This work describes the use of magnetoresistive (MR) sensors coupled with a portable platform for the detection of dengue virus (DENV), a mosquito-borne infection endemic to low- and middle-income countries, affecting 100-400 million people each year1. Nowadays, diagnosis relies on molecular or serological tests. Still, these tests remain discrete (RNA vs. antibodies), and tend to be complex and/or expensive, requiring expert staff and fully equipped laboratories. As such, this work aims to combine both assays in a single analysis by simultaneously detecting DENV RNA and anti-DENV antibodies using a portable platform2,3. A spin-valve based biochip was designed and functionalized with specific DNA oligo probes and secondary antibodies (Fig1A)4. Extracted RNA and serum samples were provided by INSA-CEVDI and used as targets in molecular and serological assays, respectively. In both assays, streptavidin coated 250 nm magnetic nanoparticles were used for biolabeling. The measurement output is represented by a voltage drop (dV/V) proportional to the number of captured target molecules (Fig1B). However, to increase the dynamic range of the assays, the percentage difference of dV/V signals obtained using different immobilization strategies/probes, was taken as the final output signal. Using the MR-platform, individual tests were carried out for the detection of DENV RNA and anti-dengue human antibodies. The RNA target was detected down to a few hundreds of pM (Fig2A). For the serological response, a calibration curve with an LOD of 1.38 nM was achieved. Infected patients’ serum was also tested for anti-DENV antibodies, obtaining a sensitivity of 100% and a specificity of 92%(Fig2B). Finally, simultaneous detection of antibodies and RNA was successfully achieved on the same chip, showing no differences to the individual assays. To increase the multiplexing capability of the assay, a biochip with 144 sensors and multi-level contact layers was developed and is under testing using DENV as a model disease. This chip design will allow the simultaneous discrimination of various viruses endemic in the same regions.

References:

1 Who.int. 2022. Dengue and severe dengue. online Available at: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue Accessed 14 June 2022.
2 J. Germano et al., Sensors 9, 4119 (2009).
3 V. C. Martins et al., Biosensors and Bioelectronics 24, 2690 (2009).
4 D. C. Albuquerque et al., Biosensors and Bioelectronics 210, 114302 (2022).

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