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Cell Sorting has become an indispensable technique for a variety of biological and biomedical applications. Moreover, with the development of fields such as personalized medicine and new breakthroughs in cancer treatments, cell sorting devices that have high throughput and single-cell manipulation capabilities are needed now more than ever1. Magnetically activated cell sorting (MACS) has quickly become one of the most widely used techniques due to its extremely high performance in throughput and ease of use, unfortunately these systems lack the much needed single-cell resolution that modern biomedical problems require. To address this, there has been much research and development aimed at creating MACS devices in the microfluidic regime where microscale fabrication can allow for single-cell sorting mechanisms to be implemented. In addition to increased resolution, these microfluidic MACS devices have other benefits that include low cost, the ability to run fast low volume experiments, and the ability to capture and release cells while simultaneously culturing them within a device. In this paper we present a novel method of capturing and releasing magnetically activated cells using Spin Orbit Torque (SOT) to change the magnetization of micromagnet structures patterned within a microfluidic channel. SOT has been shown to be a reliable and fast method of manipulating the magnetization of magnetic memory devices2 and for this reason it was chosen as the method of operation. This device is a significant improvement on current cell sorting technologies because it has both single cell manipulation capabilities and high throughput whereas most current cell sorting technologies suffer a tradeoff between these two properties. This paper includes both modeling and experimental data to verify the theoretical mechanism proposed.References: 1X. Wang, S. Chen, D. Sun. Lab on a Chip., Vol. 11, p. 3656 (2011) 2W. Kwak, J. Kwon, B. K. Cho. Scientific Reports.,Vol 8 (2018)

MMM 2020

High Throughput Magnetic Single-Cell Sorting Using Spin Orbit Torque

The 65th Annual Conference on Magnetism and Magnetic Materials (MMM 2020) was held in a virtual form. The COVID-19 pandemic, with its associated uncertainty about international travel and the ability to gather in large groups, meant that the Steering Committee decided to cancel the face-to-face meeting that had been planned in Palm Beach, Florida November 2-6, 2020.

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Realizing efficient spin-charge conversion is an important issue in spintronics, especially for the development of low-power-consumption magnetization switching. A spin-charge conversion is generally induced in a nonmagnetic (NM) layer by the inverse spin Hall effect. The spin-charge conversion efficiency, so-called the spin Hall angle θSHE, is limited by the spin-orbit interaction of the NM layer. Thus, artificially enhancing the θSHE to greater than its intrinsic value is difficult. Here, using spin pumping measurements on all-epitaxial single-crystalline heterostructures composed of ferromagnetic (FM) Co2FeAl0.5Si0.5 (CFAS) and an NM n-type Ge, we demonstrate that the θSHE can be strongly enhanced by annealing the sample and the resulting interdiffusion of the atoms within only ~3.7 nm around the CFAS/n-Ge interface. The magnitude of the electromotive force was significantly different between the as-grown and annealed samples as shown in Fig. 1. From these results, θSHE is estimated to be 0.0058–0.0079 for the as-grown sample and 0.015–0.019 for the annealed one at 70–300 K (Fig. 2), which is much larger than the intrinsic value of the θSHE reported for Ge (0.00096–0.002) [1-3] and is comparable to θSHE values reported for Pt. This enhancement is attributed to strong scattering of the spin current in the intermixed layer formed at the interface by annealing. Our results indicate that the θSHE is strongly influenced by the atomic structure of the FM/NM interface, which suggests a new and efficient method to control spin-charge conversion by precise control of the FM/NM interface [4]. This work was partly supported by Grants-in-Aid for Scientific Research (No.18H03860, 19H05616), CREST of JST (No. JPMJCR1777), and the Spintronics Research Network of Japan (Spin-RNJ).References: [1] M. Koike et al., Appl. Phys. Express 6, 023001 (2013). [2] A. Jain et al., Phys. Rev. Lett. 109, 106603 (2012). [3] J. C. Rojas-Sánchez et al., Phys. Rev. B 88, 064403 (2013). [4] S. Kaneta-Takada et al., submitted (2020).

Artificial enhancement of the spin Hall angle of Ge using the interdiffusion of atoms in Co2FeAl0.5Si0.5/n-Ge heterostructures

Spin Hall nano-oscillators (SHNOs) have the unique ability to convert a direct current input to microwave signals by means of spin Hall effect and spin orbit torque [1]. The generated microwave signals depend on several factors inherent to having a flow of direct current such as the current density, Oersted fields, Joule heating and naturally the magnetic state in a given device geometry. In addition, other factors such as frequency locking or synchronisation onto an external microwave source can contribute to the microwave output of a given SHNO [2-5]. The interplay of all these factors affects the agility of the magnetisation auto-oscillations of an SHNO. We report the study of SHNOs while subject to current pulses as well as RF pulses, measured using time resolved micro-focused Brillouin light scattering (micro-BLS). The SHNOs under test consist of a double-disk constriction of NiFe(5 nm)/Pt(7 nm). First, we discuss how few-nanosecond pulses can still efficiently induce magnetisation auto-oscillations, thereby demonstrating that both the onset and outset of the auto-oscillations occur within a sub-nanosecond timescale. Then, we proceed to showing how auto-oscillations can be affected by external microwave pulses. Various degrees of enhancement (injection-locking) or suppression of the auto-oscillation signal can be achieved by choice of the frequency and the amplitude of the external microwave signal. The knowledge of the agility or the response to either intended or unwanted parasitic external excitations is paramount for SHNOs to be able to operate on very short time-scale with well defined responses in, for example, the context neuromorphic computing [6].References: [1] Demidov, V. E. et al., Nature Materials, 11(12), 1028–1031 (2012). [2] Demidov, V. E. et al., Nature Communications, 5(1), 3179 (2014). [3] Sato, N. et al. Physical Review Letters, 123(5), 057204 (2019). [4] Hache, T. et al., Applied Physics Letters, 116(19), 192405 (2020). [5] Hache, T. et al., Applied Physics Letters, 114(10), 102403 (2019). [6] Zahedinejad, M. et al., Nature Nanotechnology, 15(1), 47–52 (2020).

Agility of spin Hall nano-oscillators measured using time-resolved micro-BLS

The efficient detection of a magnetic state at nanoscale dimensions is important for the development of spin logic devices. Magnetoresistance effects can be used to detect magnetic states, but they cannot produce sufficiently large signals when the dimensions of the devices are reduced to the nanoscale, nor do they generate an electromotive force that can be used to drive a circuit element for logic device applications.1 In this work2, we report a favorable scaling law for the detection of an in-plane magnetic state of a magnet by using the inverse spin Hall effect in cobalt–iron/platinum (Co50Fe50/Pt) nanostructure devices. By reducing the dimensions of the device, we obtain a large spin Hall signal of 0.4 Ω (0.3 Ω) at 10 K (room temperature) (see figure 1a), and quantify an effective spin-to-charge conversion rate for the ferromagnetic/heavy metal nanostructure system (see figure 1b). Finally, we predict that this spin-orbit detection of magnetic states could be used to drive spin logic circuitsReferences: [1] S. Manipatruni, D.E. Nikonov, C.C. Lin, et al., Nature 565, 35–42 (2019). [2] V.T. Pham*, I. Groen*, S. Manipatruni et al., Nat.Electron. 3, 309-315 (2020)

Spin-Orbit Magnetic State Readout in Scaled Ferromagnetic/Heavy Metal Nanostructures

Since it is important to find materials with large spin Hall angles for utilizing spintronic devices, various materials have been reported, e. g. Ir–O, Pt-Cu, Ta50W50, and BiSb [1-4]. We investigated the composition effect on spin Seebeck coefficient in YIG/Pt-based alloy systematically, where Pt-based alloy is Pt1−XRuX, Pt1−XCuX, and Pt1−X(Cu0.5Ru0.5)X. The additive elements of Cu and Ru have equally small spin Hall angles in the single state, whereas they have the different atom sizes. In all systems the composition of around 50 at% showed the maximum spin Seebeck coefficient, from which we estimated spin Hall angles [5, 6]. As a result, the spin Hall angle was increased from 0.1 to 0.19, 0.25, 0.34 by changing Pt to Pt-Ru, Pt-RuCu, and Pt-Cu. It means that additive Cu is more effective on spin Hall angle improvement than additive Ru although their own original spin Hall angles are comparably small. X-ray diffraction analysis revealed that Cu and/or Ru doping in Pt host decreased the unit cell size, and there is an obvious relation between the lattice constant and the spin Hall angle as shown in Fig. 1(a). Since the resistivity dependence on lattice constant exhibit the similar tendency in Fig. 1(b), it suggests that the unit cell size modification in heavy metal Pt layer increases the electron scattering including spin-dependent scattering, leading the extrinsic spin Hall angle enhancement.References: [1] K. Fujiwara, Y. Fukuma, J. Matsuno, H. Idzuchi, Y. Niimi, Y. Otani, and H. Takagi, Nat. Commun. 4, 1 (2013). [2] R. Ramaswamy, Y. Wang, M. Elyasi, M. Motapothula, T. Venkatesan, X. Qiu, and H. Yang, Phys. Rev. Appl. 8, 024034 (2017). [3] H. Yuasa, F. Nakata, R.Nakamura and Y. Kurokawa, J. Phys. D: Appl. Phys. 51, 134002 (2018). [4] N. H. D. Khang, Y. Ueda and P. N. Hai, Nat. Mater. 17, 808 (2018). [5] K. Tian and A. Tiwari, Sci. Rep. 9, 1 (2019). [6] H. Li, Y. Kurokawa, T. Niimura, T. Yamauchi, and H. Yuasa, Jpn. J. Appl. Phys. 59, 073001 (2020).

Spin Hall Angle Control by Doping Element into Pt Host

Ferromagnetic metals and insulators are widely used for generation, control, and detection of magnon spin signals. Most magnonic structures are based primarily on either magnetic insulators or ferromagnetic metals, while heterostructures integrating both are less explored. Here, by introducing a Pt/yttrium iron garnet (YIG)/permalloy (Py) hybrid structure grown on Si substrate, we studied the magnetic coupling and magnon transmission across the interface of the two magnetic layers [1]. Interestingly, we found the YIG and the Py layers show antiferromagnetic coupling when external field is small, and the two layers align to the same direction only when the in-pane field is above 150 mT, as evidenced by both vibrating-sample magnetometry and polarized neutron reflectometry measurements. More importantly, the parallel and antiparallel magnetization configurations in this YIG/Py structure could be utilized to control magnon spin flow in spin-pumping and spin Seebeck experiments. In Figure 1(a) and 1(b), we schematically illustrate the effect of magnetization configuration (antiparallel vs parallel) on the magnon current transmission efficiency across the two magnetic layers’ interface. In the spin-pumping experiment, as plotted in Figure 1(c), we demonstrate that a magnon spin-valve device with an ON/OFF ratio of ~130% can be realized out of this multilayer structure at room temperature. Owing to the efficient control of magnon current and the compatibility with Si technology, the Pt/YIG/Py hybrid structure could potentially find applications in magnon-based logic and memory devices.References: [1] Yabin Fan, P. Quaterman et al., Phys. Rev. Appl. 13, 061002 (2020).

Manipulation of coupling and magnon transport in magnetic metal-insulator hybrid structures

The focus of lab-on-a-chip technology centers on small, low power devices that can be integrated on a microscopic scale to perform laboratory functions within one unit or “chip”. In order to achieve this, one has to understand how to transfer fluid at this scale. Due to the low Reynolds number limit, such devices need to break time-reversible symmetry to produce a net flow. There has been a degree of interest and research into using magnetic materials to achieve this. In this work, we propose a new model employing ferromagnetic elements for construction of a practical microfluidic pump. The design is based on a repeated chain of magnetic discs connected together via links with hinges at the centers of the discs, which allow the discs to rotate (Fig 1a). While the model is based on that of ferromagnetic swimmers[1]. The device is surrounded by fluid and is activated by an external AC magnetic field. The magnetic and elastic forces in this device can work together against the fluidic forces to create a time-irreversible movement, which results in a net flow. While magnetisation of all the discs are oriented in the same direction, the relative rotational stiffness of the discs (or hinge stiffness) is varied to produce a lag in the response to the magnetic torque. This collective effect is similar to that of a metachronal wave, which is ultimately responsible for the flow. We found that if both discs had the same rotational stiffness, then no flow was produced. Depending on the chosen frequency, there is an optimal rotation stiffness of the two discs where a maximum flow rate is achieved. We observed as the frequency increases so does the flow, until the frequency is too large for the discs to turn to their maximum angle before the direction of the magnetic field reverses. To conclude, we demonstrated that an elasto-magnetic device can produce a net flow at low Reynolds number. The flow direction can be controlled by altering the frequency of the driving magnetic field or by using different rotational stiffness. These results could lead to new types of ferromagnetic pumping devices.References: [1] A. D. Gilbert, et al., Q. J. Mech. Appl. Math., 64, 239 (2011).

Investigation of a Coupled Elasto-Magnetic Discs for Low Reynolds Number Pumps

Introduction: Transcranial magnetic stimulation (TMS) is an emerging method for depression and neuropathic pain therapy with a painless and noninvasive neuromodulation function [1]. The winding path of the TMS coil can be realized in several ways [2]. For instance, the H1 coil is designed for deep brain stimulation [3], the “hat-like” coil is designed to suppress temperature rise during stimulation [4], and the fdTMS coil comprehensively considers energy, depth, and focality [5]. However, a practical TMS coil satisfying both high energy efficiency and robustness against individual variabilities in head shape has not yet been realized. Purpose: To optimize an energy-efficient TMS coil that is robust against individual differences in head shape. Methods: The optimization scheme is shown in Fig. 1. Multiple MRI anatomical images are used to optimize the shape to facilitate fitting of the scalp at the target stimulation position. Subsequently, the winding path of this shape is optimized through the following steps. First, the forward model Ε=AΨ for the scalar stream function Ψ and target-induced electric field Ε is established. Second, the Tikhonov regularization is used to solve the stream function Ψ numerically, given Ε. Finally, the coil winding path is extracted from the contour lines of Ψ [4]. This coil was compared with two commercial TMS coils by simulating the eddy current distribution in the digital brain model. Result: The results are shown in Fig. 2. Under the same energy consumption condition, compared with planar fig-8 and butterfly coils, this “head-fit” coil has stimulus area increases of 87% and 67%, respectively, with the corresponding efficiency drops of only 2% and 3%. The expansion of the stimulation area indicates that stimulation to the target area can be ensured even with a slight movement of the head during ~30min treatment. Furthermore, considering the shape of the scalp of different subjects facilitates close fitting of the head. Conclusion: Our method allows the design of TMS coils suitable for practical TMS therapy.References: [1] J. P. Lefaucheur et al., “Evidence-based guidelines on the therapeutic use of repetitive transcranial magnetic stimulation (rTMS),” Clin. Neurophysiol., vol. 125, no. 11. Elsevier Ireland Ltd, pp. 2150–2206, 2014. [2] Z. De Deng, S. H. Lisanby, and A. V. Peterchev, “Electric field depth-focality tradeoff in transcranial magnetic stimulation: Simulation comparison of 50 coil designs,” Brain Stimul., vol. 6, no. 1, pp. 1–13, Jan. 2013. [3] H. M. Gellersen and K. K. Kedzior, “Antidepressant outcomes of high-frequency repetitive transcranial magnetic stimulation (rTMS) with F8-coil and deep transcranial magnetic stimulation (DTMS) with H1-coil in major depression: a systematic review and meta-analysis,” BMC Psychiatry, vol. 19, no. 1, pp. 139, 2019. [4] L. M. Koponen, J. O. Nieminen, and R. J. Ilmoniemi, “Coil optimisation for transcranial magnetic stimulation in realistic head geometry,” Brain Stimul., vol. 10, no. 4, pp. 795–805, 2017. [5] L. J. Gomez, S. M. Goetz, and A. V. Peterchev, “Design of transcranial magnetic stimulation coils with optimal trade-off between depth, focality, and energy,” J. Neural Eng., vol. 15, no. 4, pp. 1–31, 2018. [6] G. N. Peeren, “Stream function approach for determining optimal surface currents,” J. Comput. Phys., vol. 191, no. 1, pp. 305–321, 2003.

Optimization of transcranial magnetic stimulator coil using stream function for reducing the influence of individual variabilities of head geometry

Recently, we have introduced a new type of magnetic particles (MPs), prepared by wet milling of superferromagnetic Fe-Cr-Nb-B precursor glassy ribbons, for cancer treatment by magneto-mechanical actuation in low magnetic fields (1÷20 Oe) [1]. In this work we studied the possibility of using adipose derived stem cells (ADSCs) for the transport of Fe-Cr-Nb-B MPs to areas with MG-63 human osteosarcoma (HOS), and the effect of MPs incorporated into ADSCs on the viability of HOS following the magneto-mechanical actuation of MPs with dimensions between 5 and 100 nm. The viability tests performed 24 hours after the magneto-mechanical actuation by MTT assay indicated values of 24÷25% for HOS and up to 10% for ADSCs. We also investigated the capacity of ADSCs to incorporate Fe-Cr-Nb-B MPs, and we found that large amounts of MPs were ingested as confirmed by a colorimetric ferrozine-based assay that quantitatively evaluates the Fe content in cells. We used a time-lapse cell imaging method to record the cellular migration of ADSCs loaded with MPs towards tumor cells in vitro. The recorded films demonstrated that MPs-loaded ADSCs were able to target osteosarcoma cells. We performed in vitro tests, where ADSCs were incubated with Fe-Cr-Nb-B MPs, then transferred onto an osteosarcoma cell culture and exposed to the rotating magnetic field. Magneto-mechanical actuation led to the destruction of ADSCs and to the release of MPs on HOS culture, which incorporated the released MPs. By magneto-mechanic actuation, the HOS viability dropped down to 21÷22%. The irregular shape of the MPs combined with their rotational motion in the rotating magnetic field produce irreversible damages on the cells membranes and organelles, and, subsequently, the cells death. Work supported by the Nucleu Programme (Project PN 19 28 01 01) and Contract No. 11PFE/16.10.2018 financed by the Romanian Ministry of Education and Research.)References: [1] H. Chiriac, E. Radu, M. Tibu, et al., Sci. Rep. 8 (2018) 11538.

Fe-Cr-Nb-B magnetic particles and STEM cells, triggers for cancer cells apoptosis by magneto-mechanical actuation

Magnetically actuated micro- or milli-scale gripper robots have been widely studied as a promising means to manipulate a variety of small objects, such as biological cells, medical drugs, and other functional materials, in an untethered and uncontaminated manner [1-3]. In this research, we propose a magnetic gripper robot (MGR) composed of a simple flexible body with three permanent magnets inserted into different parts of the body (Fig. 1). A cube magnet is fixed into the base of the flexible body, whereas two freely rotatable cylindrical magnets are inserted into each gripper arm of the body. Without an external magnetic field, the two cylindrical magnets in the gripper arm tend to align in the same lateral direction, which can make the MGR form a closed posture due to the attractive magnetic force. Under a specific magnetic field, on the other hand, the two magnets may align into a vertical direction parallel to the fixed magnet, in which the MGR can make an open posture due to the repulsive magnetic force. In this research, we derived an equation that can calculate the equilibrium postures of the MGR's magnets under an external magnetic field and the corresponding structural deformation of the flexible body by using the MGR’s magnetic and elastic potential energy (Fig. 1). From this equation, we could establish a method to generate the MGR’s gripper motions and its two-dimensional (2D) translational motion at the same time by using an efficient coil system comprised of only a triad of electromagnetic coils (TEC) shown in Fig. 2a [4]. We then fabricated a prototype MGR and a TEC to demonstrate the proposed MGR’s gripper motions such as the pick-and-place motions shown in Fig. 2. The results showed that the proposed MGR could play a role as an effective untethered gripper which can be utilized for various biomedical purposes such as cell manipulation, drug delivery, biopsy, etc.References: [1] T. R. Ger, H. T. Huang, W. Y. Chen, and M. F. Lai, Lab on a Chip, Vol. 13, p. 2364-2369 (2013) [2] E. Diller and M. Sitti, Advanced Functional Materials, Vol. 24, p. 4397-4404 (2014) [3] T. Xu, J. Zhang, M. Salehizadeh, O. Onaizah, E. Diller, Science Robotics, Vol. 4 (2019) [4] H. J. Lee and S. M. Jeon, AIP Advances, Vol. 10 (2020)

A Two-Dimensional Magnetic Gripper Robot Driven by a Triad of Electromagnetic Coils

The blood's acid-base balance is precisely controlled because even a minor deviation from the normal range can severely affect many organs. Due to Warburg effect, in tumor cells, an imbalance between oxygen supply and consumption, and accumulation of lactate cause increasing the concentration of H+. As a consequence, intracellular pH decreases, resulting in acidity. Since the pulsed magnetic field (PMF) influences human physiology, many studies have been reported its effect on anti-nociception as well as improvement of blood circulation[1-2]. Our study has tried to investigate the influence of PMF on blood's acid-base balance in microvascular channels. In addition, the applicability of PMF to H+ pump inhibitor in tumor cells was evaluated using negatively- charged magnetic beads(MB). Tert-butyl hydroperoxide (tBHP) was used to oxidizing agents, in order to make acidic environment similar to tumor cells. All experiments were done with red blood cells(RBCs) and plasma suspension with hematocrit (Ht) of 45%. Our PMF stimulator has the maximum intensity of 0.27T at a transition time of 102 μs with pulse intervals of 1Hz. The changes in pH of blood and the number of RBCs with MB attached were examined before and after PMF stimulus. The blood treated with tBHP on RBCs is more acidic than that of normal blood, but it was observed that the pH is restored after PMF stimulus. Also it was confirmed that MB adheres more to the RBCs after PMF stimulus, which could be inferred that PMF stimulus not only increases the zeta potential, but also reduces the H+ in the blood by combining MB with plasma cations. Further study needs to optimize PMF stimulation conditions such as pulse shape, duration, or repetition rate for fast homeostasis recovery in acid-base unbalanced blood.References: [1] N. M. Shupak, F. S. Prato & A. W. Thomas, Neurosci. Lett. Vol. 363, p.157–162(2004). [2] J. Mok, S. Han, & H. Lee, AIP Advances, 10(1), 015005 (2020).

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