United States

South Korea’s National Post-Market Surveillance (NPMS) program annually collects and tests approximately 1,650 marketed veterinary chemical drugs under formulation- and ingredient-based plans. Eighteen public authorities including 17 provincial governments and the Animal and Plant Quarantine Agency (APQA) collect products, while a testing laboratory designated by APQA has performed all active ingredient assays since 2018.
The Efficacy &amp; Adverse-Effect Monitoring (EAEM) program complements NPMS by retesting manufacturer-retained products from NPMS noncompliants and investigating complaints submitted through public portals and repeat-offender targeting. Though separately operated, the two systems are linked by a risk-tiered feedback mechanism.
From 2018 to 2024, NPMS tested 11,579 products, detecting 193 non-compliances (1.7%), mainly powders (42%) and injectables (31%). Frequent noncompliances involved vitamin A, dexamethasone, spiramycin, amoxicillin, colistin, and florfenicol. Retained-product retesting confirmed 87 noncompliants (58.8%), but the system was abolished in 2024 due to growing concerns over the reliability of equivalence between retained and marketed products in some manufacturers.
In 2024, following the phase-out of retained-collected product testing, additional tests focusing on formulation-specific quality features such as sterility, pH, and disintegration were conducted. EAEM also performs regular tasks such as contract lab proficiency testing, LC-MS/MS cross-contamination screening, bioequivalence trials, disinfectant efficacy evaluation (ASF, AI, FMD), and veterinary diagnostic kit performance tests.
Through structured NPMS sampling, EAEM verification, and targeted pharmacovigilance inspections, Korea has maintained a noncompliance rate below 2% for veterinary chemical drugs, and uses NPMS and EAEM data to guide GMP oversight and improve regulatory policy.

2025 AOAC INTERNATIONAL Annual Meeting &amp; Exposition

Post-Market Surveillance and Risk-Based Quality Assessment of Veterinary Chemical Drugs in South Korea (2018–2024): Seven-Year Data Analysis and Regulatory Follow-Up Mechanism

#### The AOAC INTERNATIONAL Annual Meeting: A Unique Analytical Science Opportunity

The AOAC Annual Meeting & Exposition provides unparalleled professional development, networking, and collaboration in methods-based science. 

* **Businesses**: Meet scientific and regulatory experts and engage with new trends and standards.

* **Scientists**: Build professional expertise and network with your community to share information and best practices.

* **Regulators**: Leverage unprecedented opportunity for stakeholder collaboration on complex testing and analysis challenges, helping improve compliance and public safety.

* **Academia**: Engage with colleagues – both students and faculty – at all career and research levels.


#### Keep Pace with Innovation
* Take advantage of emerging methods, best practices, and trending topics like food fraud and cannabis purity and potency. 
* Discover new technologies, from DNA authentication to genomic microbial identification. 
* Get professional insights from industry insiders through the AOAC Spotlight.

#### Experience the AOAC Analytical Solutions Forum

This multi-faceted “idea incubator” focuses on regulatory changes and emerging food safety issues. A plenary and two breakout sessions feature thought-provoking ideas to identify and meet analytical needs.

The 2025 AOAC INTERNATIONAL Annual Meeting & Exposition was held in person in San Diego, USA.

<br><br>

You need to log in with the email address you registered with. 



Please log in to explore this event.

To access this event, you need to register [here](https://www.aoac.org/2025-annual-meeting-exposition/).



Please register!

2025 AOAC INTERNATIONAL Annual Meeting & Exposition

The AOAC Annual Meeting & Exposition provides unparalleled professional development, networking, and collaboration in methods-based science.

South Korea’s National Post-Market Surveillance (NPMS) program annually collects and tests approximately 1,650 marketed veterinary chemical drugs under formulation- and ingredient-based plans. Eighteen public authorities including 17 provincial governments and the Animal and Plant Quarantine Agency (APQA) collect products, while a testing laboratory designated by APQA has performed all active ingredient assays since 2018.
The Efficacy & Adverse-Effect Monitoring (EAEM) program complements NPMS by retesting manufacturer-retained products from NPMS noncompliants and investigating complaints submitted through public portals and repeat-offender targeting. Though separately operated, the two systems are linked by a risk-tiered feedback mechanism.
From 2018 to 2024, NPMS tested 11,579 products, detecting 193 non-compliances (1.7%), mainly powders (42%) and injectables (31%). Frequent noncompliances involved vitamin A, dexamethasone, spiramycin, amoxicillin, colistin, and florfenicol. Retained-product retesting confirmed 87 noncompliants (58.8%), but the system was abolished in 2024 due to growing concerns over the reliability of equivalence between retained and marketed products in some manufacturers.
In 2024, following the phase-out of retained-collected product testing, additional tests focusing on formulation-specific quality features such as sterility, pH, and disintegration were conducted. EAEM also performs regular tasks such as contract lab proficiency testing, LC-MS/MS cross-contamination screening, bioequivalence trials, disinfectant efficacy evaluation (ASF, AI, FMD), and veterinary diagnostic kit performance tests.
Through structured NPMS sampling, EAEM verification, and targeted pharmacovigilance inspections, Korea has maintained a noncompliance rate below 2% for veterinary chemical drugs, and uses NPMS and EAEM data to guide GMP oversight and improve regulatory policy.

Non-targeted methods have been gaining ground in recent decades, especially with the tremendous development of high-resolution mass spectrometry. The challenges for non-targeted methods are becoming increasingly necessary as there are limited guidelines to regulate and harmonize the methods worldwide. AOAC-Europe and have been signed a Memorandum of understanding for this sector. Two years ago, a series of Webinars on "Trends & Challenges for Non-Targeted Methods" have been started. As a result of this webinars a joint working group was set up with the aim of providing guidelines for the harmonization of non-targeted methods, mainly based on Mass Spectrometry. So far, the starting working group was now split into five subgroups dealing with food, authenticity, environmental/water, packaging and metabolomic testing. As an initial step the five groups are summarizing definitions and workflows for non-target testing. Based on the theses results a harmonized wording and where possible a harmonized workflow incl. validation should be created. The aim of this presentation is to present especially the result of the packing (food contact material area). The current status of initiation of common parameters as well harmonized criteria for reporting Screening (NIAS) results is presented. The focus of this harmonisation activity is on descriptive physico-chemical parameter, which allow the reader of such Screening reports to find out easily, if it worthwhile to compare results or are the test results achieved based on different assumptions.
Currently the focus is only on the results but not on comparability, which lead to massive problems in understanding.

Harmonization on Non-targeted Testing in Mass Spectrometry

Non-targeted analysis (NTA) is a type of analytical technique with the purpose of identifying chemicals in a material without the use of a priori knowledge regarding the chemical composition of the material. As novel methods using NTA approaches rapidly emerge, the Best Practices for Non-Targeted Analysis (BP4NTA) Working Group formed to address the need for harmonization across research communities. The presentation will discuss all facets of BP4NTA, from formation in 2017 to its current goals. Over the past 8 years, BP4NTA has become an international, nonprofit organization consisting of members from academia, government (state, federal US, and non-US), and industry with a range of NTA experience. Initial efforts within BP4NTA aimed at harmonization of language and methods through the creation of the informative website with NTA Reference Content. Numerous other outcomes have been generated, ranging from manuscripts and tools to engagement activities with varied stakeholders involved in the development of NTA workflows and use of the data. Examples include the NTA Study Reporting Tool, guidance on understanding performance metrics for non-targeted analysis and proposing a roadmap for the high-impact research directions for NTA. In addition, BP4NTA has identified opportunities fulfill an educational in training and supporting researchers that are new to NTA. Moving forward BP4NTA endeavors to identify new collaborative opportunities with other scientific communities using non-targeted approaches, develop new tools that further enhance transparency and reproducibility of non-targeted analysis, and to establish best practices for scientists using non-targeted approaches.

The Best Practices for Non-Targeted Analysis (BP4NTA) Working Group

Per-and Polyfluorinated Substances (PFAS) are diverse synthetic chemicals widely used in industry because of their thermal stability, hydrophobicity, and relative inertness. These “forever chemicals” have been detected in food and food packaging and may cause harmful health effects. With thousands of PFAS compounds identified, processing non-targeted PFAS data poses a significant challenge.
In this study, 40 paper-based food contact materials (FCMs) from shops in Montreal, Canada -- including to-go boxes, microwave popcorn bags, wrappers, paper straws, and baking liners -- were analyzed for the occurrence of PFAS using LC-QTOF MS. The highest PFAS concentrations were detected in clamshell to-go boxes, with levels reaching up to 356.6 ng/g. PFOA (up to 187.2 ng/g) and PFDA (up to 92.2 ng/g) were major contributors to the overall PFAS content. FluoroMatch Modular was used to annotate previously unidentified PFAS, confirming a homologous series  (C3 to C14) of perfluoroalkyl carboxylic acids (PFCA) and C6 and C8 perfluoroalkyl sulfonic acids (PFSA). 
The study also demonstrated that the migration of both short-chain (PFHxA and PFHpA) and long-chain (PFOA, PFDA, PFNA) PFAS into food simulants increased upon heating (65 ℃), indicating that higher temperatures facilitate the release of these substances from packaging into food. These findings raise concerns about potential for PFAS exposure through food consumption, particularly under heated conditions such as hot meals and microwave heating.
The widespread detection of PFAS in clamshell to-go boxes and other FCMs in this study highlights the need for more stringent regulations and oversight to minimize PFAS use in FCMs.

Non-Targeted Analysis of PFAS in Food Contact Materials: Unveiling Hidden Dangers with LC-QTOF MS and FluoroMatch

NMR spectroscopy is an incredibly powerful analytical tool that provides a wide array of techniques for conducting metabolomics studies. It has become an essential tool in for assessing quality, authenticity, safety, and geographical origin. However, several challenges hinder its broader application in food research, such as low sensitivity, signal overlap, and the predominance of sugar signals that obscure important signals of non-sugars. To overcome this limitation, we report the implementation of past reported sugar-suppression methods combined with these conventional standard 1D and 2D NMR methods. This approach minimizes the sugar signals and allows for clearer detection of other metabolites. Total sugar and non-sugar sixty-eight metabolites were identified, and three-fold enhanced intensities and visibility of non-sugar signals in suppressed 1D 1H NMR and 2D NMR methods compared to their respective standard  NMR methods. Combining NMR-based metabolomics with multivariate analyses like PLS-DA and heat maps helps identify compositional variations in honey samples, aiding in the discrimination of botanical origins. . Glucose, cellobiose, fructose, galactose, glucose-6-phosphate, maltose, mannitol, ribose, sucrose, trehalose, uridine diphosphate (UDP)-galactose, UDP-glucose, adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine triphosphate (ATP), acetate, asparagine, betaine, formate, gallate, glutamate, glutamine, malonate, hydroxymethylfurfural, phenylalanine, pipecolate, and [AA1] proline are main responsible primary metabolites that contributed to botanical origins discrimination. Interestingly, the applied suppressed methods improved signal intensity, enabling clearer identification of metabolites amidst complex carbohydrate backgrounds. This approach also facilitated the detection of vitamins like nicotinic acid and trigonelline, emphasizing the nutritional value of the honey samples by reducing interference from dominant carbohydrate signals.
  

Untargeted Metabolomics of Honey of Four Different Botanical Origins by Conventional and Sugar Suppressed 1D and 2D NMR Spectroscopy

Dietary fiber is recognized as an essential part of human nutrition due to its health benefits. As consumers become more aware of these benefits, there is a growing interest in products with higher dietary fiber content. This underscores the need for accurate measurement of dietary fiber in food products for labeling and other purposes. Over the past decades, our understanding of dietary fibers has evolved, with new types of fibers emerging, such as those derived from algae, insects, pectin, and hemicellulose. This evolution necessitates the optimization of existing analytical methods or the development of new ones to keep up with advancements and cover various types of dietary fiber. Consequently, numerous analytical methods have been developed to determine dietary fiber content, making accurate testing more complex. 
This contribution briefly discusses the differences between the most important analytical methods for dietary fiber determination, highlighting some recently developed solutions for novel fiber ingredients and addressing some shortcomings in current methods.

Unraveling the Fiber Frontier: Innovations and Challenges in Dietary Fiber Analysis

Supplements are known globally by various names like Dietary Supplements (DS), Health Food (HF), Food Supplements (FS), Natural Health Products (NHP), and Complementary Medicines (CM). Each category must meet local market standards for compliance, leading to differing regulations based on product classification.
This session will cover a case study on the challenges and gaps in the Nutritional Fact Panel (NFP) analysis of dietary supplements, with examples from Canada and Australia markets to illustrate issues in meeting international requirements. NFP (Nutritional Facts Panel) is an analysis that requires a combination of parameter assessment. For the dietary supplements, these details are required to report on the product label by regulation which again is classified by different terms from different regulatory bodies around the world. In Canada, this is referred to as NFT (Nutritional Fact Table) as classified by Health Canada and Canadian Food Inspection Agency (CFIA). In Australia, the authority known as Food Standards Australia New Zealand (FSANZ) refers to it as the Nutritional Information Panel (NIP). Part of this NFT disclosure, calorie estimation is a critical parameter required to indicate product/formula attributes. The standard methodology for calorie estimation, as outlined by the United States Department of Agriculture in the “Energy Value of Foods,” Agricultural Handbook, and FDA 21 CFR Part 101.9 for food versus 21 CFR 101.36 for supplements, involves analyzing carbohydrates, dietary fiber, fats, protein, cholesterol, ash, moisture, and iron in any product.
This session will conduct a structured review to identify specific challenges associated with gaps in current analytical methods. It will also address how a product development approach may result in biased data interpretation to examine the regulatory requirements that impact accurate labeling. 

A Case Study: Challenges in Analyzing Nutritional Facts Panels of Dietary Supplements for Compliance with Global Regulations

AOAC 2017.16, for the measurement of total dietary fiber, in alignment with CODEX definitions, achieved final action status in 2020, and replaced AOAC 2009.01 as a CODEX Type 1 method in 2021.  AOAC 2022.01, for the measurement of soluble, insoluble and total dietary fiber is following a similar track with AOAC final action status obtained in 2024 and it is expected to replace AOAC 2011.25 as a CODEX Type 1 method this year. As these methods are implemented across the industry, new insights into analytical challenges continue to emerge. This presentation investigates chromatography-related questions, particularly the performance of recommended columns (Waters SugarPak, AOAC 2009.01; Tosoh TSKgel, AOAC 2001.03/2017.16/2022.01) alongside alternative columns sourced globally. A comprehensive carbohydrate dataset was analysed across these columns to evaluate their performance. The comparative chromatographic performance results will be discussed herein.
Additionally, regulatory developments in pet food labelling are driving a transition from crude fiber to dietary fiber analysis, as signalled by the Association of American Feed Control Officials (AAFCO). By 2029, pet food labelling will require dietary fiber determination by approved analytical methods, necessitating a major shift in analytical approaches. To support this transition, we generated relevant data using these methods, assessing their applicability to pet food matrices, the results of which will also be described.

Recent Developments in Dietary Fiber Methodology

Last decade novel carbohydrate dietary or prebiotic fibre are being developed. Products are being sent for novel food applications (Gras, Efsa, ..). To meet acceptance criteria and enable commercialization new and highly accurate and discriminative carbohydrate quantification methods are needed. Using traditional gravimetric-LC carbohydrate, sugar and fibre methods are not capable of providing accuracy and specificity to support novel food dossiers for toxicity and stability testings as well composition testing in foods. One would need new methods to do so. Calculation of carbohydrates by 100-minus methods no longer will do.
Examples of novel carbohydrate ingredients are milk oligosaccharides, fermentation based carbohydrates, algae based carbohydrates, hemicellulose based oligosaccharides (AXOS), and pectin based oligosaccharides (POS) and foods made thereof.
Analogous to accepted proteomic and amino acid profiling approaches also glycomic or so-called carbohydrate composional profiling based methods are developed using modern UHPLC-FLD-MS methods. This presentation will show not only the suitability in scientific research and the developement of the Davis Food glycopedia or glycan nutrition encyclopedia but also the potential as validated reproducible QA methods.

New QA Test Methods for Carbohydrate Prebiotic Novel Food Ingredients

Functional mushrooms are rapidly gaining popularity in the natural products market, driving the need for improved botanical identity testing. Traditional chemistry-based methods can struggle with accurate identification due to overlapping chemical profiles and environmental variability within species and tissue type. This challenge is further compounded in blended formulations, where precise identification can become even more difficult. This talk will demonstrate how DNA-based approaches overcome these challenges to showcase how DNA testing can be a powerful option for accurate species identification despite the growing conditions, tissue type, product type, or blend formulation. Case studies on Lentinula edodes (shiitake) and Cordyceps militaris (cordyceps) will illustrate successful application of DNA testing across various product matrices, including finished goods and complex blends. By integrating DNA-based authentication into quality control, the industry can establish a strong option for mushroom verification, ensuring accuracy, transparency, and trust from cultivator to consumer.

Genetic Verification of Functional Mushrooms: A Reliable Approach to Ingredient Testing in Finished Products

Introduction:
Postbiotic materials have immense growth potential in the supplement industry (11.3% CAGR 2024-32). However, the inactivation process limits downstream QC methods since traditional plate count methods are inapplicable. Digital PCR (dPCR) is a technique that can be employed to accurately and precisely count postbiotics, provided the dPCR enumeration methods are validated and fit for purpose.
Purpose: 
To validate a dPCR method for postbiotics and establish accuracy of the method by comparison with a reference value (flow cytometry).
Method: 
Postbiotic Lacticaseibacillus paracasei raw material was validated according to AOAC Appendix K parameters and acceptance criteria. Parameters included specificity, precision/intermediate precision, accuracy, and robustness. For accuracy, low, medium, and high concentrations of L. paracasei blended with excipient were analyzed by dPCR and flow cytometry for comparison with an acceptance criterion of 70-125% recovery.
Results: 
The dPCR method for the enumeration of postbiotic L. paracasei passed the acceptance criteria for all parameters of the validation. Closely related specificity strains had no dPCR detection, whereas the eight-strain blend spiked with L. paracasei had 90% recovery, passing the specificity criterion. The low, medium, and high concentration L. paracasei products had an average of 94, 81, and 91% recovery, respectively, compared to flow cytometry, thus meeting accuracy criterion.
Significance:
dPCR is a powerful tool for postbiotic quantification. This validation demonstrates the method’s reliability and suitability for QC release testing in third-party laboratories.

Next from 2025 AOAC INTERNATIONAL Annual Meeting & Exposition

Harmonization on Non-targeted Testing in Mass Spectrometry

Similar lecture

Pesticides in California’s Adult-Use Cannabis Market: Prevalence, Analytical Challenges and Concerns, and Regulatory/Market Solutions

Stay up to date with the latest Underline news!

PRESENTATIONS

CONFERENCES

COMPANY

RESOURCES