Would you like to see your presentation here, made available to a global audience of researchers?
Add your own presentation or have us affordably record your next conference.
Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals widely used in industrial and consumer products. As dietary exposure to PFAS through foods become a growing concern, the AOAC SMPR 2023.003 provides specific guideline for developing analytical method for PFAS analysis in various food matrices. Although alcohlic drinks are not included in the list, the PFAS residue in alcoholic drinks becomes a concern and thus requires a reliable analytical method for PFAS determination in alcoholic drinks. In this study, we developed and validated a workflow for 43 PFAS compounds analysis in alcoholic drinks using QuEChERS extraction followed with EMR mixed-mode passthrough cleanup, and then direct injection of sample eluent for LC-MS/MS detection. The inject program utilized a novel injection mode called feed injection, where sample is fed into LC gradient with excellent mixing with initial mobile phase condition. The feed injection enables direct injection of large volume sample in high organic, delivering excellent chromatography without compromise on early eluted targets' peak shape. The improved instrumetn method further simplified the sample preparation by removing the drying step, delivering the acceptable method LOQ levels but saving up to 50% of preparing time. The method achieved LOQs less than 0.01 µg/kg for the core four PFAS targets and ≤ 0.1 µg/kg for other PFAS targets, except PFBA and PFPeA, which have < 1 µg/kg LOQ being demonstrated. Method recovery and reproducibility, selectivity and suitability were evaluated based AOAC SMPR guideline and demonstrated to meet the acceptance criteria.
