Chlorate and perchlorate contamination in agricultural produced foods and their extracts has become a growing concern for regulatory agencies and consumers due to associated health risks. Chlorate contamination mainly occurs through the use of chlorinated water or chlorine-containing detergents during cleaning and sanitizing process on farms and at processing plants. Perchlorate is a naturally occurring contaminant in the environment and can be introduced to agricultural produced foods through contaminated groundwater or the use of specific natural fertilizers.  Given the potential health implications of chronic exposure, developing robust methods for routine monitoring of chlorate and perchlorate levels in food products is essential. This presentation summarizes a single laboratory validation (SLV) study for a liquid chromatography tandem mass spectrometry (LC-MS/MS) method designed to accurately quantify chlorate and perchlorate in various challenging food matrices including fruits, vegetables, cereals, leaves, flowers, rhizomes, and their extracts. The validation of this test method reported here on aspects of specificity, precision, accuracy, linearity, range, limit of detection (LOD), limit of quantitation (LOQ), and ruggedness for both analytes. Validation involved analysis of spike all food matrixes at least two concentration levels including LOQ and 10x LOQ level with at least five replicates per concentration level. This robust method offers cost-effective approach to quantifying chlorate and perchlorate in complex food matrixes using direct extraction techniques.