Ammonia is produced via protein catabolism of ingested food in the gastrointestinal tract (GIT), resulting in total ammonia (Tamm) levels in the chyme up to 50-fold higher than blood ammonia levels. Chyme ammonia also far exceeds the water ammonia levels considered toxic for fish. Thus, the high luminal ammonia concentration must impose a challenge if ammonia is transported across the gastrointestinal epithelium into the bloostream, but relatively little is known about this possibility. To address this, we monitored Tamm of blood supplying and leaving the GIT in freshwater rainbow trout (Oncorhynchus mykiss) over 48 h following feeding. We also investigated possible NH3 transport across GIT epithelia by measuring Rhesus glycoprotein mRNA expression levels (as possible NH3 transporters) and ammonia flux rates in in vitro in response to experimentally varied PNH3 gradients. We found that the subintestinal venous blood Tamm levels near the posterior intestine remain undisturbed after a meal and differ markedly from those in the lumen. Although Rhesus glycoproteins appear to be present selectively in different regions, PNH3 may not be a significant driver for ammonia transport into the bloodstream. However, high rates of ammonia absorption occurred in the stomach in vitro and Tamm in the hepatic portal vein, which collects blood from the whole GIT, increased following feeding. Overall, we found that in addition to the intestine, the stomach is involved in ammonia assimilation and that each GIT region may have distinct roles in ammonia metabolism and transport (NSERC Discovery).

In And Out Of The Gastrointestinal Tract of Trout: Selective Transport Of High Luminal Ammonia Into The Venous Drainage

[![](https://assets.underline.io/uploads/markdown_image/1/image/68d3f4f3b018b6e483ae67310c4ef296.png)](https://zoom.us/rec/play/YkI6yKMGYZuQTFfEAn16bFAwxnZeHxnu4waXaAzEa5jmd18GGi2kilSnNXOG9dGU3e9Zod0Ajph5T2cZ.mnoHECOKx0m3VIjW?autoplay=true&startTime=1657090497000) <br>

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A23:  Open Animal

technical paper

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2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years - Montpellier, France

Ellen Hyewon Jung

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In And Out Of The Gastrointestinal Tract of Trout: Selective Transport Of High Luminal Ammonia Into The Venous Drainage

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