Testing how evolutionary cold adaptation affects metabolism and performance of fish we compared the acute responses to warming of the stenothermal Antarctic eelpout Pachycara brachycephalum and of the eurythermal common eelpout Zoarces viviparus from the North Sea. We investigated whole animal oxygen consumption rate and ammonia excretion. As excretion of ammonia via the gills reflects whole animal metabolic patterns, especially nitrogen and amino acid metabolism, we also investigated the temperature dependent protein synthesis rate of gills in vivo and connected it to respiration and ammonia excretion. 
Both species were exposed to temperature changes within their species-specific thermal window:  P. brachycephalum -acclimated to 0°C- at a rate of 2°C day-1 until 10°C, and Z. viviparus - acclimated to 4°C - at a rate of 3°C day-1 until 22°C. Once temperature stabilized (within 12 hours), oxygen consumption rates were measured repeatedly and water samples were taken to determine the ammonia excretion of each fish individually. In a parallel experiment, we investigated the temperature dependent protein synthesis rate (Ks) of gills after intraperitoneal injections of 13C-labeled phenylalanine. The gill tissue was sampled 1.5 and 3 hours after the injection.
All measured parameters followed an exponential increase with warming for both species, however, with different Q10 values (e.g. Q10 protein synthesis rate P. brachycephalum 4.2 vs. 2.8 Z. viviparus). Protein synthesis rates at the same temperatures were similar in both species (P. brachycephalum vs. Z. viviparus: 4°C Ks= 2.5 vs. 2.6 % day-1; 10°C Ks= 4.5 vs. 4.3% day-1) indicating no cold compensation regarding protein synthesis in gills of the Antarctic eelpout. It will be discussed how these results are related to oxygen uptake and ammonia excretion.

The impact of acute warming on the respiratory system of fish – a comparison of eelpouts from Antarctica and temperate zones

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2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years - Montpellier, France

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The impact of acute warming on the respiratory system of fish – a comparison of eelpouts from Antarctica and temperate zones

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