Ischemic stroke is a devastating neurological disease caused by a reduction in blood flow to the brain that results in neuronal cell death. Subsequent neuroinflammation is mediated by central nervous system resident immune cells and peripheral immune cells. Evidence suggests that stroke-induced gut dysbiosis results in priming of pro-inflammatory immune cells in the intestine that migrate to the stroke lesion and contribute to neuroinflammation. Currently, it is unknown whether gut dysbiosis and the migration of gut-derived immune cells to the stroke lesion persist in the chronic phase following injury. In this study, we aimed to characterize; (I) stroke induced gut dysbiosis and (II) the contribution of gut-derived immune cells to neuroinflammation, over time in a rodent model of ischemic stroke. Using 16s rRNA sequencing we observed that gut dysbiosis is observed at D4 following injury in both males and female animals. Interestingly, females exhibit a return to baseline microbial composition at D20 while males continue to exhibit gut dysbiosis. In addition, we injected a lipophilic cell-tracking dye (Cm-DiI) into peyer's patches at various timepoints following injury to observe whether gut derived immune cells migrate to the brain beyond D3. Using immunohistochemical analyses, we observed Cm-DiI+/CD45+/DAPI+ cells in the stroke injured hemisphere at D4 and D9 following ischemic stroke induction. These findings suggest that stroke induced gut dysbiosis and the migration of gut-derived immune cells to the stroke lesion are phenomena that persist beyond the acute phase of injury.

Characterizing gut dysbiosis and the gut derived immune response to ischemic stroke

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PS-3-C -  Disorders of the Nervous System

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Colleagues and Friends:

I am very happy to welcome you to the 14th annual Canadian Neuroscience Meeting, and to the first virtual edition of our meeting. I want to highlight the important work the CAN meeting chair, **Roger Thompson** and co-chair, **Alyson Fournier**, did to put together the great program we are offering this year. Moving to a virtual format required a complete re-design of the program, and both Roger and Alyson did a splendid job in providing us with an appealing and well adapted program for our first virtual event. I wish to thank them for this very important effort.

Highlights of this year’s scientific meeting include invited speakers **Michael Hausser, Franck Polleux, Gwyneth Card, Magdalena Götz**and Brain Prize winner **Huda Zoghbi**. Plenary and parallel symposia, proposed by our members complete our diverse [scientific program.](https://can-acn.org/meeting-2021/program-2021/)

The 2021 Advocacy session, chaired and organized by CAN advocacy committee chair **Karun Singh**, will feature a special lecture and question period with Member of Parliament and past Science Minister **Kirsty Duncan**. This will be followed by a short overview of CAN’s advocacy activities over the last year. We invite you to participate in this session for insight from Dr. Duncan on the workings of Parliament Hill, and to learn more about what CAN has been doing to promote neuroscience research in Canada.

We are also excited to host **Lisa Willis** in the Equity Diversity and Inclusion session, organized by EDI committee chair **Jibran Khokhar**.

A special highlight of our meeting will be the lectures by award-winning young neuroscientists. We are please to host the CAN 2020 Young Investigator Award winner **Mihaela Iordanova**, and CAN 2021 Young Investigator Award winners **Aaron Phillips** and **Tabrez Siddiqui**. Highlights will also include presentation by the top three winners of the CAN-CIHR-INMHA Brain Star Award winners, **Claire Gizowski, Nuria Daviu Abant** and **Lukas Tamayo-Orrego**.

Independently organized satellite symposia complement and complete this exciting program, check them out here: https://can-acn.org/meeting-2021/2021-satellite-symposia/

Some of the most engaging sessions are always the poster sessions featuring the work of our trainees, so make sure to log in to Gather Town during the poster sessions to chat with the presenters.
We hope you enjoy this program, and look forward to welcoming you, virtually.

Shernaz Bamji

President of the Canadian Association for Neuroscience

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Julia Copeland

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Characterizing gut dysbiosis and the gut derived immune response to ischemic stroke

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