Current hypotheses propose that sleep/wake regulation depends on both the circadian clock and a homeostatic process. The glycogen synthase kinase-3 beta (GSK3b) regulates the activity of key circadian transcription factors (e.g., BMAL1, CLOCK). GSK3b is also involved in long-term depression and metaplasticity. Moreover, FXR1, a GSK3b substrate, has recently been shown to affect both cell-autonomous homeostatic plasticity and system-level response to sleep loss in a GSK3b-dependent manner. This project thus aims to investigate a potential role of GSK3b in circadian and homeostatic sleep/wake regulation by verifying how its knockdown (KD) in cortical neurons impacts vigilance states under normal (baseline: BL) and sleep-deprived (SD) conditions. A neuron-specific KD of Gsk3b was achieved in adult male mice via dual adeno-associated virus delivery of a CRISPR/Cas9 system. Cortical viral injections and electrocorticography (ECoG)/electromyography (EMG) electrodes implantation surgeries were done simultaneously 3 weeks prior to ECoG/EMG recording, which comprises 24 h of BL, 6 h of SD, and 18 h of recovery. Analyses notably include vigilance state duration/distribution and ECoG spectral activity. Gsk3b KD did not affect the time spent in wakefulness and sleep states nor the alternation between states. However, the KD of Gsk3b in the motor cortex decreased gamma (30-50 Hz) activity during wakefulness and alpha (8-10 Hz) activity during slow-wave sleep (SWS). Moreover, a SD-induced increase in beta/gamma (20-50 Hz) activity during SWS was found in the visual cortex only under Gsk3b KD. These results support a role for GSK3b in the regulation of ECoG activity during wakefulness and sleep, and bring a deeper insight on molecular mechanisms controlling vigilance state quality.

Knocking down glycogen synthase kinase-3 beta (GSK3b) in cortical neurons affects the mouse electrocorticogram across vigilance states

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PS-1-F -  Cognition and Behavior

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Colleagues and Friends:

I am very happy to welcome you to the 14th annual Canadian Neuroscience Meeting, and to the first virtual edition of our meeting. I want to highlight the important work the CAN meeting chair, **Roger Thompson** and co-chair, **Alyson Fournier**, did to put together the great program we are offering this year. Moving to a virtual format required a complete re-design of the program, and both Roger and Alyson did a splendid job in providing us with an appealing and well adapted program for our first virtual event. I wish to thank them for this very important effort.

Highlights of this year’s scientific meeting include invited speakers **Michael Hausser, Franck Polleux, Gwyneth Card, Magdalena Götz**and Brain Prize winner **Huda Zoghbi**. Plenary and parallel symposia, proposed by our members complete our diverse [scientific program.](https://can-acn.org/meeting-2021/program-2021/)

The 2021 Advocacy session, chaired and organized by CAN advocacy committee chair **Karun Singh**, will feature a special lecture and question period with Member of Parliament and past Science Minister **Kirsty Duncan**. This will be followed by a short overview of CAN’s advocacy activities over the last year. We invite you to participate in this session for insight from Dr. Duncan on the workings of Parliament Hill, and to learn more about what CAN has been doing to promote neuroscience research in Canada.

We are also excited to host **Lisa Willis** in the Equity Diversity and Inclusion session, organized by EDI committee chair **Jibran Khokhar**.

A special highlight of our meeting will be the lectures by award-winning young neuroscientists. We are please to host the CAN 2020 Young Investigator Award winner **Mihaela Iordanova**, and CAN 2021 Young Investigator Award winners **Aaron Phillips** and **Tabrez Siddiqui**. Highlights will also include presentation by the top three winners of the CAN-CIHR-INMHA Brain Star Award winners, **Claire Gizowski, Nuria Daviu Abant** and **Lukas Tamayo-Orrego**.

Independently organized satellite symposia complement and complete this exciting program, check them out here: https://can-acn.org/meeting-2021/2021-satellite-symposia/

Some of the most engaging sessions are always the poster sessions featuring the work of our trainees, so make sure to log in to Gather Town during the poster sessions to chat with the presenters.
We hope you enjoy this program, and look forward to welcoming you, virtually.

Shernaz Bamji

President of the Canadian Association for Neuroscience

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Tanya Leduc

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Knocking down glycogen synthase kinase-3 beta (GSK3b) in cortical neurons affects the mouse electrocorticogram across vigilance states

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