Common Bean (Phaseolus vulgaris) provides protein, complex carbohydrates, and other valuable nutrients for more than 300 million people in the tropics and around the world. Common bean is particularly known for its ability to provide fiber, with some studies showing that it aids in the lowering of blood sugar. It is also used as a model species for more complex plant species, as it is easy to grow and make genetic crosses for studying gene linkage. In this project, the goal was to identify if there was a significant difference in the quality and quantity of DNA isolated from fresh versus frozen leaf tissue. Fresh, young, trifoliate leaves were directly collected from newly grown common bean plants for DNA isolation using CTAB (Cetyltrimethylammonium bromide) buffer to help break down leaf tissue. The same procedure was used with common bean leaf tissue that had been stored in the -80 degrees deep freezer for more than five years. Differences in color, texture, and ease of grinding the leaf tissues were some physiological comparisons identified between fresh and frozen tissue. Both isolated DNA were run on a gel electrophoresis to visualize results. Of two fresh and frozen samples each, the results indicate that the frozen samples were more concentrated by visualization as well as quantification with a Nanodrop. Ultimately, the frozen tissue provided higher concentrations of DNA. Keywords: Common bean, DNA, CTAB, Gel Electrophoresis, Nanodrop Acknowledgements: We acknowledge USDA NIFA Capacity Building Grant Awards# 2016-06642 and 2016-06476 for funding my internship and the project as part of the student experiential learning goal and the Project Director, Dr. Gulnihal Ozbay for providing this internship opportunity.

Does Prolonged Storage of Plant Tissue have a Negative Effect on DNA Quality?

Posters - Pre-College

poster

Welcome to Delaware State University’s Annual Summer Research Symposium, and thank you for attending! Our symposium represents the culmination of students’ summer experiences in research at Delaware State University. This event showcases the work of college students working on faculty-led research projects, as well as middle and high school students experiencing research for the first time working in research groups. Whatever the case, this summer has been a unique experience for the students, and we hope a transformative one. Even for students who are not planning a career in scientific research, their summer experience has given them skills they will draw on for years to come. 
 Please join us on the mainstage and in Gather for the live event, and visit the recorded poster presentations any time.
 The student participants and organizers of the various summer programs at DSU would like to thank all of the research mentors who guided our summer program participants. There were many DSU faculty, graduate students, and other research and program staff who gave their time and energy to help our students have a fun and educational summer experience. Without their generosity and patience these summer programs would not be possible.
 For the research participants, we hope that you will maintain contact with your research mentors and colleagues. Science is a relatively small community made up of disciplinary “neighborhoods”. If you continue to work in a discipline, the other folks in that discipline will be like your neighbors that you run into regularly. Staying in contact with them will help you keep up with the news of the neighborhood, and open doors to educational and career opportunities. Whether you continue in science or take a different career path, for this summer you have been part of the world of research, and you will benefit from that perspective for the rest of your life.

Please register for the DSU Summer Research Symposium 2021 to be able to access the content.  
If you have already registered, please login with the same email you used to registered for the Conference.

DSU Summer Research Symposium 2021

Welcome to Delaware State University’s Annual Summer Research Symposium, and thank you for attending!

Dashawn Williams-Bey

1

SHORT BIO

Presentations

Does Prolonged Storage of Plant Tissue have a Negative Effect on DNA Quality?

Stay up to date with the latest Underline news!

PRESENTATIONS

CONFERENCES

COMPANY

RESOURCES