Czechia

Awarding gaps are differences in degree classification between demographic groups. In the UK there are known awarding gaps on the basis of ethnicity, socioeconomic status, age and disability. Progress on closure of gaps is notoriously slow, with inequity persisiting year after year. In this presentation I will explore the potential of innovative assessment in closing gaps. At the University of Hull we have made significant progress in closing awarding gaps, primarily through an institution wide focus on innovative, authentic competency based assessment. I will give an overview of this at institution level and within the biosciences, and provide practical suggestions for equitable assessment design

SEB Conference Prague 2024

Closing Awarding Gaps through Innovative Assessment: A whole university approach

awarding gaps

undergraduate education

equity

technical paper

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Thermal acclimation represents a key process enabling ectotherms to cope with changing environmental conditions, including climate change or biological invasions. Intraspecific variations in thermal acclimation may reflect adaptation to locally prevalent environmental conditions. Environmental gradients can provide useful models to study such processes. Here, we compared the thermal acclimation capacity of the invasive common periwinkle Littorina littorea, along a large latitudinal gradient. Specifically, we exposed adult snails collected from 10 different locations along Atlantic North America to 12 temperatures (5 to 27 C) and measured survival, feeding rate, growth, metabolic rate and Ctmax.
Our results showed that latitude, average summer temperature, and growing season length at the place of origin influenced snails’ thermal responses. In particular, snails from higher latitudes showed a higher capacity for acclimation, with increasing CTmax and feeding performance at higher temperatures. Furthermore, survival underwent a steeper decrease towards higher temperatures for snails from lower latitudes, showing they possess a better acclimation capacity in colder locations. In addition, growth and metabolic rate curves presented differences among snails from different locations. In conclusion, feeding, CTmax and survival results were coherent with a counter-gradient adaptive scenario. Low-latitude individuals may be lacking acclimation capacity because they already live near their thermal limits, while higher latitudes individuals may possess higher acclimation capacity to better exploit the shorter periods of warm temperatures they experience. Whereas growth and metabolic rate results indicate the presence of potential local adaptation, especially at the distribution edges, characterised by harsher conditions. 


Beat the heat. Thermal acclimation across latitudes reveals counter-gradient physiological variations in a widespread marine gastropod.

Amphibians are resilient species that have adapted during their long evolutionary history to rather diverse environmental conditions. Current climate change, however, is pushing many species tolerance limits, especially as it is translating into not only increased temperature but also, increased drought and shortened pond duration. Some species have evolved the ability to adaptively alter their phenotype in response to some of these factors, but the combination of hazards and the rate at which they are taking place, may exceed the ability of amphibians to cope. Reduced pond duration may trigger developmental acceleration in amphibian larvae, allowing them to reach metamorphosis sooner, but at the cost of doing so smaller and with depleted fat reserves. Moreover, if they metamorphose onto a harsher environment, the odds of survival during the juvenile stage may be severely reduced. We are studying the plastic responses of tadpoles of the spadefoot toad, Pelobates cultripes, to risk of pond drying, which is a complex combination of reduced water level, increased temperature, increased larval density and reduced food availability. We then also studied the carry-over effects of the developmental alterations experienced by larvae onto the juvenile stage, especially when they then face drier and warmer environments, monitoring juvenile survival, growth, activity pattern, hormonal levels and thermal tolerance.

Plastic responses of amphibians to shortened pond duration and increased drought and temperature

Insects use the adhesive force working on the soles of the legs for walking on the walls and ceilings. Particularly, fruit flies achieve stable wall walking due to the scale effect. However, the ground reaction force (GRF) during wall walking has not been measured because of lightness of their body. In this study, we use a force plate whose tilt angle can be adjusted to measure the total GRF of all legs in the shear and vertical directions when a fruit fly walks on a wall. The force plate has measuring section with an area of 10 mm^2 and spring structures that move in the shear and vertical directions, and it measures forces in two axes using Hooke's law. The resolution in both directions is 0.36 µN and 0.31 µN, which represents about 1/30 of its weight. It could be fabricated through a simple process by using thin film glass as the force plate material and processing the spring structure with a UV laser marker. It could adjust the inclination angle of the force plate via θ-axis stage, thereby the wall would be prepared. The GRF in two axis is measured when a fruit fly walks on the plate. When the force plate was placed horizontally, we confirmed that the fruit fly walked with almost no vibration in the shear and vertical directions.

Measurement of the ground reaction force of fruit fly using a tilted biaxial force plate

Telomeres are non-coding ‘caps’ on the end of chromosomes that change in length in response to growth, oxidative stress and various stressors. Due to this relationship, they have been proposed to link to organismal and cellular self-maintenance, and may be used as a biomarker of past stressors such as heat stress. Understanding how environmental temperature can impact telomere length and cellular maintenance is crucial for understanding the vast ways in which organisms' cellular and life-history processes may respond to predicted environmental warming. To further aid in validating telomeric biomarkers, we subjected two populations of Australian Painted Dragon lizards from differing ends of a temperature-aridity gradient to a three-month hot or cool thermal treatment. At the end of the experiment, we assessed the correlations of blood telomere length to four tissues (heart, liver, muscle and testes). We will discuss these results in terms of the uses and pitfalls of using telomere length as a biomarker and how the natal thermal environment and thermal treatments interact and influence telomere length.

Thermal effects on tissue telomere length in a colour polymorphic ectotherm, the role of thermal geographic variation.

I am an integrative evolutionary biologist studying the phenotypic and biogeographic impact of duplication. From single genes to entire genomes, duplications are often associated with adaptive radiations, evolutionary novelties, and decreased extinction during periods of environmental change. Most studied in plants, there are nevertheless thousands of these major duplication events across animals and fungi, with more being discovered each year. I investigated comparative biogeography of polyploid and diploid frogs in South America. I discovered a remarkable and novel trend of spatial clustering within polyploid lineages. In all five genera of South American frogs with variable ploidy, virtually all polyploid occurrences were found in the southeast, largely to the exclusion of diploids. In fact, polyploid species occur more closely with intergeneric polyploids than they do with congeneric diploids, suggesting an environment which selects for genome duplication. Polyploid occurrences were subject to greater levels of seasonal temperature fluctuation and agricultural impacts, either of which may contribute to the formation and fitness of polyploid lineages. Later, I expanded this framework into a global study encompassing amphibians, ray-finned fishes, and insects. On this scale, differences were even more pronounced between cytotypes. In each of the three clades, polypoid occurrences became more frequent with distance from the equator. I attributed this trend in large part to glaciation cycles, which were the most important model variable in all three clades

Macroecological consequences of whole-genome duplication: a latitudinal polyploid gradient in three vertebrate clades

Organisms that occupy environments contaminated by radionuclides such as Chornobyl, Ukraine, face putative detrimental effects. How radioactive pollution affects organisms, especially in early life, is not well studied in the wild. Our study focuses on two interconnected factors that might be altered due to radionuclide contamination of the environment and influence fitness: early-life diet and gut microbiota, an important physiological constituent tied closely to diet and host physiology. We quantified fitness components, diet, and gut microbiota from nestlings of two passerine birds, Great tit (Parus major) and Pied flycatcher (Ficedula hypoleuca), by sampling animals in nest-boxes located in contaminated and uncontaminated areas within the Chornobyl Exclusion Zone, Ukraine. We used amplicon sequencing to identify dietary insects and to quantify the bacterial diversity and composition of the birds’ gut microbiota. Environmental radiation had no effect on fitness components (body condition and brood size) of either species. Both species had greater diversity in dietary insects in contaminated areas. In both bird species environmental radiation was not associated with bacterial diversity of gut microbiome, however radiation was associated with composition of microbiome. Other ecological factors: forest type, brood size and age were associated with P. major gut microbiome composition but not in F. hypoleuca. Our data show comparable responses in diet and gut microbiome of two wild species when exposed to environmental radionuclide contamination. These results give valuable insight to ecological processes in contaminated environment.

Environmental contamination by radionuclides alters diet and gut microbiota of wild passerines

Gut microbiomes are integral to animal eco-physiology and are hypothesized to shape the adaptive evolution of animal life. However, directly estimating the extent to which gut microbiomes are under selection within free-living animal populations is challenging. The inheritance mechanisms that allow for the intergenerational transmission of microbiome variation within animal populations are also unclear. However, joint characterization of the inheritance and fitness consequences of microbiome variation are critical for understanding the role of microbiota in animal adaptation. To address these knowledge gaps in parallel, I quantified relationships between gut microbiomes and host survival in the wild, by using shallow shotgun metagenomic sequencing to characterize microbiota communities in archived fecal samples collected across seven years of a long-term individual-based study of Sable Island feral horses (2820 samples from 905 individuals). Using quantitative genetic animal models, I then estimated the narrow-sense heritability and social transmissibility of survival-associated microbiota. Analysis of these data indicate that horse survival may be strongly connected to methane production in the hindgut. Furthermore, quantitative genetic analyses suggest the involvement of both host genetics and social effects in structuring the microbiome, and that these effects may be strongest for the microbiota most closely connected to horse survival. By dovetailing estimates of natural selection and inheritance, these results provide a fuller understanding of how host-microbiome relationships evolve in nature.

Straight from the horse's mouth: lessons of gut microbiome selection and inheritance from Sable Island

Assessment IS learning. The power of assessment to do more than just test knowledge and understanding is substantial. Making our assessments meaningful to our students’ future lives, and authentic to the skills they will need is a major priority in the evolving Higher Education sector. Ensuring that our assessments are also student-centred, and develop their skills both as bioscientists and as independent, self-regulated learners, is also an important necessity. This talk will focus on ways in which we can enhance our assessments to ensure they are focused on supporting student learning and preparing them for their future employment needs, and will highlight the challenges associated with this.

Meaningful and student-centred assessment in Biosciences education

Temperature is a major factor governing the distribution and seasonal behavior of plants. The mild increasing ambient temperatures affect the growth rate and morphology of plants. Global warming makes it imperative to decipher how crops sense and transduce the warm temperature signals to physiological acclimations and developmental programs. We performed phenotyping on a spring barley collection consisting of 400 accessions grown at normal and mild warmer day-time temperatures and characterized the thermoresponsive traits at vegetative stages. Genome-wide association studies identified SNPs and their proximal genes associated with leaf length and tillering related traits. We examined the organ-specific transcriptome dynamics in three barley genotypes grown at two temperature conditions. The time-courses of differentially expressed genes induced by warmer temperatures reflect the varieties of thermoresponse speed, sensitivities, and acclimation in three genotypes. We also performed MNase-defined cistrome occupancy analysis (MOA-seq) to identify genomic regions with altered chromatin accessibility and transcription factor occupancy induced by warmer temperatures. Combining GWAS, transcriptome and cistrome, we identified novel regulators regulating barley thermomorphogenesis, which provides new perspectives for breeding climate-smart crops.



Feeling the warmth: the molecular mechanisms underlying the morphogenetic response of spring barley to warmer temperatures

Hypomethylation in plants is associated with improved stress responses and is therefore a favourable trait. However, when methylation mutants are produced there is often a transgenerational decrease in fitness, meaning this is not a viable avenue to explore in crops. 

Utilising a set of epigenetics recombinant inbred lines (epiRILs), where met1-3 was crossed with Col-0 to produce a ‘mosaic pattern’ in DNA methylation, we found that a region of chromosome 3 where was inheritance solely from the Col-0 parent. This region contains the gene IBM1, for which transgenerational deleterious effects has been previously associated with change in intronic methylation, leading to alternative splicing of the IBM1 transcript and ectopic genomic hypermethylation occurring at gene bodies. 
In the same regions we also found another gene, a vacuolar ATPase (VHA), had a similar methylation profile to IBM1 and complementary expression within the flower. We hypothesized that the interaction of IBM1 and VHA could explain the transgenerational decrease in fitness observed in met1-3 plants. Existing RNA-seq data was assessed for this possibility, and a range of genetic approaches were applied including crossing of IBM1 and VHA knockout mutants and CRISPR-Cas9 to generate double mutants. 
We propose there is incompatibility between the pollen and carpels of these methylation mutants, ultimately resulting in decreased fitness and viability


Closing Awarding Gaps through Innovative Assessment: A whole university approach

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Beat the heat. Thermal acclimation across latitudes reveals counter-gradient physiological variations in a widespread marine gastropod.

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Closing Awarding Gaps through Innovative Assessment: A whole university approach

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Next from SEB Conference Prague 2024

Beat the heat. Thermal acclimation across latitudes reveals counter-gradient physiological variations in a widespread marine gastropod.

Stay up to date with the latest Underline news!

PRESENTATIONS

CONFERENCES

COMPANY

RESOURCES

Downloads