Czechia

Repeated hypoxic events are reported in various brain pathologies. Mammal brain is highly vulnerable to hypoxia, because brain functions require high oxygen and energy demands. Marine mammals, such as pinnipeds, were naturally selected to cope with daily repeated hypoxic events during foraging dives, when human brain can only cope with minor repeated hypoxia. How do pinnipeds cope with repeated severe hypoxic events at the blood-brain interface? We performed a comparative physiological approach using for model the Southern Elephant Seal (SES), which is the most extreme diver of all seals and being exposed to severe peripheral hypoxia.
Immunolabelling of various blood-brain interface markers (endothelium, astrocytes, neurons) were performed on brain cortical slices from freshly dead post-weaning pups (n=4) and reproductive adults (n=5) collected at Kerguelen Island. Immunolabelling were visualised using confocal microscope and images were analysed using ImageJ. 
Cerebral vascularisation exhibits significant differences between pups and adults: 1) in the grey matter, the vascular volume fraction was around 62% higher in adults than in pups; 2) the vessels were significantly more ramified and tortuous in adults (e.g. 6% more tortuous in the white matter); 3) some large blood vessels exhibited abnormalities in adults suggesting blood-brain interface dysfunctions. Interestingly, adult SES vessels share similar phenotypes to pathological cerebral-blood vessels described in human neurodegenerative diseases. Then, abnormal vessel-morphologies raise the question of the potential pathological situation for adult SES. To study blood-flow velocity in brain-vessels and premature aging in SES, biomechanical fluid modelling, additional staining for neurodegenerative landmarks and blood-biomarkers are currently performed.

SEB Conference 2024

Does cerebral blood vessel phenotype represent and adaptative response to repeated hypoxia in the southern elephant seal?

tortuosity

brain vascularisation

immunohistochemistry

mirounga leonina

hypoxia

neurodegeneration

# Welcome everyone to ACL 2022!

The 60th Annual Meeting of the Association for Computational Linguistics is taking place May 22-27, 2022 as a hybrid event, in Dublin and online. We are happy to welcome all of you to this anniversary edition with an almost 50-50 in-person and virtual participation. 
The main conference program features oral presentations, in-person and virtual posters and demo sessions, a plenary session for our best paper presentations and awards, three amazing keynote events and two new initiatives of invited talks: Spotlight Talks for Young Rising Stars (STIRS) and The Next Big Idea Talks. Posters (including Findings of ACL 2022) and demos are grouped by areas for both the in-person and the virtual sessions. For the virtual component, the talks will be on Zoom and the posters and the demos will be in GatherTown. The Student Research Workshop will have an oral session and a poster session as part of Poster Session 1. The program also features eight Tutorials and 28 Workshops. 

 
We wish you a wonderful conference! 
[**The ACL 2022 Organizing Committee**](https://www.2022.aclweb.org/organisers)
 
[**Conference Handbook**](https://drive.google.com/file/d/1_BUCMfhMVrjG9E2e71aHdHeE28KSje0l/view?usp=sharing) 
[**Mini Handbook**](https://drive.google.com/file/d/1qlBKl0wzmlVF1oCeMQl3BahLd9nLP5Ce/view?usp=sharing) 
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ACL 2022

The Association for Computational Linguistics (ACL) is the premier international scientific and professional society for people working on computational problems involving human language, a field often referred to as either computational linguistics or natural language processing (NLP). 

technical paper

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SEB’s Annual Conference is celebrated for creating the perfect composition of research, new discoveries and collaborative connections. Be creative and innovative by exchanging ideas and knowledge with masters of biology from all over the world.

In recent years, human activities in German waters have expanded continuously, impacting native harbour porpoise populations (Phocoena phocoena). Harbour porpoises often display severe pathological lesions in the respiratory tract, mainly caused by bacteria and parasites. Additionally, telemetry studies revealed they do not use their complete lung volume. It has not been studied whether this is due to the pathological lung lesions reducing the oxygen uptake of the porpoises, impairing their diving ability. 
Using multi-omics, this study aims to investigate whether harbour porpoises developed compensatory molecular adaptations to an inadequate oxygen uptake, therefore staying competitive. 
Multi-omics of non-healthy harbour porpoise lungs revealed an enhanced response to reactive oxygen species such as hydrogen peroxide known to cause oxidative stress. Potent detoxifiers such as multiple S100A genes and glutathione peroxidases were upregulated and highly abundant. Also, an extensive activation of immune system responses against microbes, bacteria and other organisms was identified. Elevated chemotaxis hints at a swift aggregation to the site of infection or inflammation, while mucosal immune responses and transport suggests quick pathogen removal. Moreover, upregulation of surfactant proteins may aid in immunoprotection and respiration of the impaired lung tissue. Reduced cell adhesion, tissue remodelling and apoptosis execution may avoid further damage by suspending cell-cell interactions and cell death.
These insights suggest a comprehensive immune response to pathogens, inflammation and reaction to ROS, enabling specific functional analyses of newly identified transcripts. Further, this study illustrates the potential of multi-omics to noninvasively advance the knowledge of marine mammal adaptations, especially to anthropogenic impacts.

Integrative multi-omics approach reveals a possible adaptation of harbour porpoise lung tissue as response to damage

Climate warming is pushing aquaculture species to their physiological limits through elevated temperature pressures impacting feed intake, nutrient use, growth, health, and welfare. Emerging research shows that diet (and dietary supplements) can play a significant role in modulating the stress response and improving thermal tolerance, which is pivotal in aquaculture species as the oceans continue to warm. 5-aminolevulinic acid (5-ALA), is an emerging dietary supplement that is the sole precursor to heme and fundamental for the formation of hemoproteins involved in aerobic energy metabolism (haemoglobin, myoglobin, cytochrome c oxidase) and the antioxidant system (catalase). Using juvenile barramundi as a model species, a feed trial was conducted for 6 weeks utilising 5-ALA supplementation in aquafeeds at 3 inclusion levels (control (0 ppm), 15 ppm, 30 ppm). Water temperature was ramped up from 30°C to 37°C, over 4 weeks to determine the diet effects under chronic thermal stress conditions. Although haemoglobin levels and catalase activities remained similar among treatments, growth rates increased with 5-ALA supplementation. 5-ALA supplementation also increased mitochondrial respiration in Oxidative Phosphorylation (OXP) with substrates supporting Complexes I and II. However, oxygen flux in the Leak (non-phosphorylating) respiration state remained similar, indicating that the Leak/OXP ratio decreased with 5-ALA. Therefore, the capacity and apparent efficiency to synthesise ATP had increases with 5-ALA. Overall, as a dietary supplement 5-ALA holds promise in aquafeeds through increasing mitochondrial efficiency, which may account for the observed increases in growth rates, which can improve production efficiency during periods of environmental stress within the aquaculture industry.

Enhancing mitochondrial efficiency: small dietary changes improve fish growth under chronic thermal stress

Climate warming with associated heat waves presents a concerning challenge for ectotherms such as fishes. During heat waves, the ability to rapidly acclimate can be crucial for survival. However, surprisingly little is known about how different species and life stages vary in their acclimation dynamics, including the change in thermal tolerance through acclimation (i.e. acclimation capacity), the duration needed for the acclimation temperature to significantly alter thermal tolerance (i.e. acclimation response time), or the duration needed to achieve full acclimation (i.e. time to full acclimation). To shed light on this knowledge gap, we studied the acclimation dynamics of three wild-caught fishes (goldsinny wrasse, three-spined stickleback and European flounder) by assessing upper thermal tolerance (CTmax) after different periods of time acclimating to a warmed environment, and measured both CTmax and lower thermal tolerance (CTmin) in juvenile and adult lab-bred zebrafish acclimated to warmed or cooled environments. Thermal tolerance of zebrafish and sticklebacks significantly increased after a three hour exposure to a warm treatment, while this took six and 24 hours in wrasses and flounders, respectively. Goldsinny wrasse had the highest acclimation capacity, and did not reach full acclimation and stabilization of CTmax within the duration of the study. All other species fully acclimated between 4–10 days. Furthermore, juvenile zebrafish showed similar acclimation dynamics to adults for both upper and lower thermal tolerance, but had a higher CTmin for all acclimation durations. Our results demonstrate that acclimation dynamics vary across species, but may be similar between life stages within species. Understanding species-specific thermal plasticity is important for accurately modeling the projected impacts of climate change.

Capacity for rapid thermal acclimation differs between life stage and species in fish

The remarkable mechanical properties of spider silk, especially its extensibility and strength, have long fascinated naturalists and engineers alike. Recent advancements have focused on unravelling the structural hierarchies and biochemical compositions that confer these unique properties, paving the way for biomimetic applications in materials science and engineering. In this scenario, spider capture threads pose a unique challenge, as many spiders produce metastructures, which unknown properties depend on both the mechanical characteristics of the fibres composing them, and their geometrical arrangement.
In this work, we focused on the biomechanics of the capture threads of the Southern house spider (Kukulcania hibernalis) employing a combination of high-resolution tensile testing, microscopic examinations, and theoretical modelling. Our findings reveal the critical role of the hidden lengths provided by a unique hierarchical looped fibre architecture, which enhance the thread’s extensibility. Thanks to the theoretical modelling, we reveal how the outstanding extensibility observed in the capture threads of Kukulcania can be explained independent of the nano-structure of the fibres. Furthermore, our research highlights the potential of spider silk meta-structures as a model system for developing fibre-based materials that mimic biological structures for enhanced mechanical performance.


Extreme elongations of Kukulcania hibernalis threads thanks to looped meta-structures

Gastropods use adhesive locomotion to successfully traverse a range of substrates by adapting the properties of a thin mucus layer. Sample collection methods can disrupt the native mucus structure, so to investigate this system effectively a method for visualising the native deposition of gastropod mucus trails in situ was developed. Optical profilometry and video analysis were utilised to uncover how animal behaviour impacts mucus structure and analysis of the dried trail topography provided insight into mucus alignment and deposition rate.

Our results revealed that dried gastropod trails have a non-uniform deposition profile with ridges visible along both edges of the trail. This phenomenon was independent of specimen size for the slug species Arion vulgaris, Arion flagellus and Limax maximus, but direction and velocity did impact the prominence of the trail ridges. The dried trail topography was the inverse of the convex deposition profile observed in the rehydrated mucus trails of a marine gastropod, indicating a potential difference in mucin deposition or alignment that is independent of the mucus hydration state. When compared to the model slug species, Ariolimax columbianus, the species from this study had thinner dried mucus trails, highlighting potential variations in the cost of adhesive locomotion between species. Our results suggest that mucus deposition rate and animal behaviour have a greater impact on mucus trail topography than species or specimen mass. Moreover, analysis of the dried mucus trails can provide invaluable insight into the relationship between the hydration and mucin costs associated with mucus excretion and adhesive locomotion.

Trail Watch: Investigating Gastropod Mucus Trails using Optical Profilometry

Male tree crickets produce advertisememt calls that are essential for mating success. Perception of this sound involves the capture of sound waves by an eardrum and mechanoreception of vibrations by sensory cells within the ear. To aid the detection of courtship calls present at low levels in noisy environments, tree crickets use active amplification within the ear. However, this sensitive system may be overwhelmed when noise is loud or similar in frequency to the call of interest. In this study, we performed a series of experiments to determine how and whether a tree cricket Oecanthus nigricornis eardrum is able to represent a call of interest in the presence of a simulated chorus of distracting calls at varying sound pressure levels and frequencies. Because anthropogenic noise has also been shown to impact the ability for many animals to acoustically communicate, we also performed experiments with white and pink noise as a distracting signal to simulate noise regimes generated by humans. This work provides insight on the mechanical functioning of cricket ears, and also on how these animals may be affected by different ecological conditions, including those generated by humans.

Tree cricket tympanal mechanics in noisy environments

Plants, like all living organisms are subjected to mechanical forces arising either from their environment or from within their own body. To sense these forces, plants like animals, have developed mechanosensitive (MS) channels.
MS channels identified and partially characterized in plants belong to the MSL (Mechanosensitive channel of Small conductance Like), MCA (Mid1-Complementing Activity), Piezo, OSCA (hyperosmolality-gated calcium-permeable) and TPK (Two-Pore K+) families. AtMSL10 from the model plant Arabidopsis, homologous to E.coli EcMscS, was the first plant channel to be molecularly identified and shown to be mechanoactivated. It is predominantly expressed in aerial organs but also in root tips. AtMSL10 catalyzes large fluxes of anions. Amongst multiple mechanical constraints generated by their environment, plants must cope with recurring mechanical stimulation produced by wind. We show that MSL10 activity is amplified by oscillatory stimulation at frequencies corresponding to wind-driven oscillations. Therefore, AtMSL10 is proposed to represent a molecular component allowing the perception of oscillatory mechanical stimulations by plants. Recently, we have also characterized a rapidly activated calcium MS channel (RMA) in Arabidopsis. We are currently investigating the role of this channel in root mechanosensing.
Finally, we will stress the need to reintegrate plant MS channel in the cellular-context. Indeed, MS channels described with the patch-clamp technique are characterized in the absence of an extracellular matrix. There is a need to map the membrane tension at the cellular scale in order to specifying where, and under which conditions, plant MS channels operate.


Mechanotransduction through force-gated ion channels in plants

The centromere is the chromosome region where the microtubules attach during cell division. In monocentric species, new centromeres can appear during evolution at an ectopic chromosomal location. A new centromere often tends to form near the progenitor centromer, and frequently, the establishment of a new centromere is accompanied by inactivation or loss of function of the old centromere. Initially, newly formed centromeres do not usually contain repeat DNAs but mature gradually through the acquisition and accumulation of repeats. As soon as one or a few repeats invade the novel centromere, their accumulation can be achieved by extended gene conversion during DSB repair. In addition to monocentric eukaryotes, which have a single localized centromere on each chromosome, there are holocentric species, with extended repeat-based or repeat-less centromeres distributed over the entire chromosome length. At least two types of repeat-based holocentromeres exist, one composed of many small repeat-based centromere units (small unit-type), and another one characterized by a few large centromere units (large unit-type). We hypothesize that the transposable element-mediated dispersal of hundreds of short satellite arrays and DNA repair formed the small centromere unit-type holocentromere in e.g. Rhynchospora pubera. The large centromere unit-type of the e.g. plant Chionographis japonica is likely a product of simultaneous DNA double-strand breaks and DNA repair, which initiated the de novo formation of repeat-based holocentromeres via insertion of satellite DNA, derived from extra-chromosomal circular DNAs (eccDNAs).

Centromere diversity - Does DNA repair drive the evolution of repeat-based holocentromeres?

Excellent research requires excellent leadership, but the goalposts for leadership excellence in life sciences are moving. Everyone regardless of seniority has leadership responsibility, and creating an accessible, inclusive, and responsive biology research environment that accurately reflects member's needs is vital for success across the sector. Despite this, it can be challenging to ensure everyone receives appropriate accommodations based on their needs. This co-created project uncovers the core tenets of inclusive leadership in biology, underlines the importance of intersectionality, and encourages participants to look beyond their own experiences and expectations of leadership. This talk explores the Inclusive Leadership Resource, shares pilot data from our self-scoring system, and highlights the importance of co-creation processes when modelling culture change in research environments. Ultimately, talk aims to equip participants with confidence, creativity, and deeper understanding when approaching intersectionality and accommodations in biological research environments.

Integrating Intersectionality: A New Lens on Leadership in Experimental Biology

Strigolactones (SLs) are a plant hormone known for inhibiting branching/tillering. Moreover, Plants release SLs into the soil to attract symbiotic mycorrhizal fungi. However, released SLs are also perceived as germination signal by seeds of root parasitic plants, such as Striga hermonthica. Structurally, natural SLs are diverse and divided into canonical and non-canonical SLs. One of the most intriguing questions in SL biology is whether all SLs fulfill the same functions as hormones in planta and simultaneously as rhizospheric signals. To address this question, we generated rice mutants lacking canonical SLs and showed that they do not exhibit the phenotypes characteristic for SL-deficient mutants, i.e. high-tillering and dwarfism. However, these mutants release exudates with a significantly reduced Striga seed-germinating activity, indicating a particular function of canonical SLs as rhizospheric signals. Furthermore, generating of rice mutants that accumulates 4-deoxyorobanchol revealed a particular function of this canonical SL as a negative regulator of shoot and panicle growth and a promoter of root growth. Taken together, our work inarguably identifies particular functions of different SL classes, opening up the possibility of targeted design of plant architecture and modulating rhizospheric communications.

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Integrative multi-omics approach reveals a possible adaptation of harbour porpoise lung tissue as response to damage

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Language in the human brain

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