In recent years, research on spider silk has surged, focusing on its mechanical, medicinal, and commercial applications. Despite this, our understanding of the behavioral utilization of distinct silk types and their functions remains limited. Moreover, there is a notable gap in methodologies to comprehend the use of spider silk glands. This study focuses on Pholcus phalangioides, commonly known as Daddy long-legs spiders, known to build three dimensional irregular webs with unique behaviours. These include using sticky prey wraps to capture airborne prey, and viscous-sticky traps to ensnare ground-dwelling prey and dragline and bridging lines for locomotion. We employed multiple methodologies on live individuals as well as silk fibres collected for in-depth understanding of silk glands uses for these behaviours. Methods included spigot morphology analysis, gland dissections for morphology, light microscopy for identifying silk fibre types, high-speed video recording for behavioral study, shock freezing to observe spider behavior under Scanning Electron Microscopy and mechanical tests for single fibres to understand their role in ecological uses. Results suggest that a multi-methodology approach offers a comprehensive understanding of silk gland behavioral use. This study provides a unique perspective beyond the prevalent focus on orb weavers, offering insights into lesser-known species. The study highlights the limitations of single techniques, emphasizing the need for a diverse methodology. Additionally, it presents a protocol applicable to studying any spider's behavioral use of silk glands.