France

In plant cells, cargo proteins destined to the vacuoles contain sequence specific information known as vacuolar sorting determinant (VSD) that is recognized and sorted by vacuolar sorting receptors (VSRs) to the vacuoles. To understand how VSR recognizes its cargos, our group have determined the crystal structures of the PA domain of Arabidopsis VSR1 in complex with the vacuolar sorting determinants (VSDs) of a protease aleurain [1] and a 12 S globulin cruciferin (CRU) 1 [2]. Structural comparison with the apo-form of VSR1-PA suggests that cargo-binding induces conformational changes in four regions of VSR1-PA. The VSDs bind to the cargo-binding loop consisting of a conserved motif of 95RGDCYF100. The invariant Arg-95 residue was found to be essential for receptor-cargo interactions and vacuolar sorting of cargo proteins. In vitro pull-down assay showed that VSR1 interacts with the C-terminal decapeptide sequences of CRU1, CRU4 and vicilin-like seed storage protein (VL) 22, but not with those of CRU2, CRU3, VL21 and VL43. Tagging the C-terminal sequences of CRU1 and VL22 to the secretary red fluorescent proteins are sufficient in redirecting the proteins to the vacuoles in Arabidopsis protoplasts. A P453A substitution converts the C-terminal sequence of CRU2 to a C-terminal VSD that is recognized by VSR1. Structural insights on receptor-cargo obtained could be useful in targeting recombinant proteins to protein storage vacuoles in seeds.

This work was supported by grants from the Research Grants  Council  of  Hong  Kong  Special  Administrative  Region,  China (CUHK14151416, C4041-18EF, AoE/M-05/12, AoE/M403/16, and C4033-19E) and The National Natural Science Foundation of China (91854201 and31670179) and by direct grants from The Chinese University of Hong Kong.

[1] Luo F. et al. (2014) “How vacuolar sorting receptor proteins interact with their cargo proteins: crystal structures of apo and cargo-bound forms of the protease-associated domain from an Arabidopsis vacuolar sorting receptor.” Plant Cell, 26, 3693-708
[2] Tsao H.E. et al. (2022) Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins. Proc Natl Acad Sci USA, 119, e2111281119.




SEB Annual Conference 2022

How cargo proteins are recognized and sorted to the vacuoles?

SUMO (Small Ubiquitin-like Modifier) modification has recently emerged as a critical class of post translational modification (PTM), playing a major role in diverse cellular pathways in all eukaryotes. A key function of SUMO is to act as a vital counterpoise to Ubiquitination, adding a layer of control above ubiquitination including competition for targets and prevention of ubiquitin-dependent protein degradation. Phosphorylation is also regulated by SUMOylation of kinases and phosphatases. Crucially, unlike ubiquitination and phosphorylation which are controlled by thousands of genes, only 33-50 genes regulate the SUMO modification pathway in eukaryotes making SUMO an ideal experimental system to decipher the language and rules of PTMs.


Discovering how SUMO transduces environmental signals into specific physiological responses will be key to understanding this new set of fundamental ‘rules of life’ that regulate processes ranging from development to disease resistance in an ever-changing environment. This 2- day satellite meeting will highlight the established cellular role of SUMO in a range of eukaryotes in development to disease along with identifying knowledge gaps and horizon scanning. Particular emphasis will be paid to emerging technologies such as single cell techniques that will be crucial to decipher the SUMO code across eukaryotes. The meeting will consist of a series of talks from established to early career researchers who are using a variety of techniques to understand SUMO mediated cellular signalling in a range of systems (including yeast, plants and animals). Each session will end in a panel discussion with the audience hosted by the chair of the session.

Sumo Modification in Cell Signalling

This 2- day satellite meeting will highlight the established cellular role of SUMO in a range of eukaryotes in development to disease along with identifying knowledge gaps and horizon scanning. Particular emphasis will be paid to emerging technologies such as single cell techniques that will be crucial to decipher the SUMO code across eukaryotes.

technical paper

Discover the art of creating extraordinary science at SEB’s Annual Conference at Le Corum Conference Center     in Montpellier, France from 5 to  8 July 2022.

SEB’s Annual Conference is celebrated for creating the perfect composition of research, new discoveries and collaborative connections. Be creative and innovative by exchanging ideas and knowledge with masters of biology from all over the world.

2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years.
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2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years - Montpellier, France

Cells typically remain relatively small, because if cells grow beyond a critical limit, the surface of the plasma membrane eventually ceases to accommodate intracellular needs. Compared to animals, plant cells can dramatically increase their size without the apparent need for surface furcation. The vacuole is the biggest plant organelle and has a space-filling function, allowing to occupy up to 90% of the cell volume (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). The dynamic regulation of vacuolar size allows plant cells to expand with little increase of the cytosol (Dünser et al., 2019; Dünser et al., 2022), maintaining a favorable cell surface to cytosol ratio during growth. Here we discuss subcellular mechanisms that ensure the coordination of cellular and vacuolar size expansions.

Coordination of growth in plants

Stomata are anatomical structures at the surface of leaves controlling gas exchanges between the plant and the atmosphere. The stomatal pore aperture is regulated by two specialized cells, the guard cells, having the capacity to reversibly change their volume and shape. The changes of the guard cells’ volume are under tight control of signaling cascades regulating the ionic transport processes across the plasma and the vacuolar membranes. In guard cells the vacuole can reversibly change its volume during the opening and closure of stomata. We have used a 3D reconstruction approach to visualize the dynamics changes subcellular compartments volumes in living guard cells. The method we developed allowed us to simultaneously quantify the volumetric modifications of the cytosol and the vacuole. Notably, we could follow modification of the subcellular organization in parallel with the ion transport dynamics at the plasma and vacuolar membranes. Our data show that the vacuole accounts for the whole guard cell volume changes. This suggests that the turgor changes of the guard cells are driven by the vacuole during stomata aperture. 

Subcellular volume changes in stomata opening: vacuolar vs cytosolic volume changes.

The centriole is an evolutionary conserved organelle that coordinates fundamental biological processes including cell division, cellular signalling and cell motility. This organelle, of 500 nm long and 250 nm in diameter is composed of about 100 different proteins, some of which have been associated with human diseases. How these proteins are organized at the level of the centriole architecture and how associated mutations could be involved in pathologies is still poorly understood. I will present the latest work from my laboratory, which tackle these fundamental structural cell biology questions using cryo-tomography, cell biology and our newly developed ultrastructure expansion microscopy (U-ExM) method.

Expansion microscopy for Structural cell biology: from centriole architecture to human diseases

Determining how plasticity of developmental traits respond to environmental conditions is a challenge that must combine evolutionary sciences, ecology and developmental biology. Developmental plasticity is defined as the ability of an organism to adjust its development depending on environmental signals, thus producing alternative phenotypes precisely adjusted to the environment. Yet the mechanisms underlying developmental plasticity are not fully understood yet. Determining how plastic developmental changes that occur in response to environmental conditions are coordinated at the physiological, cellular, and molecular levels is a challenge that must combine ecology with developmental biology. The mechanisms that underlie the development of alternative phenotypes are still unclear for many systems and is one major goal of ecological developmental biology or Eco-Evo-Devo. We observed that clownfish (Amphiprion percula) young juveniles have a different rate of white bar formation depending on the sea anemone species, their obligate symbiotic partner, in which they are recruited: white bars develop more rapidly when fish are recruited in Stichodactyla gigantea than in Heteractis magnifica. Because the sister species A. ocellaris acquire their adult color pattern during metamorphosis under thyroid hormone (TH) control, we asked whether developmental plasticity in bar formation was associated with alteration in TH status as these are the main hormones triggering metamorphosis in vertebrates. We found that thyroid hormones regulate white bar formation and that a shift in hormone levels, associated with ecological differences, results in divergent color patterns in different sea anemone species in which the young fish is recruited. Taken together our results suggest that TH control the timing of adult color pattern formation and that shifts in gene expression and TH levels are associated with ecological differences resulting in divergent ontogenetic trajectories in color pattern development. We recently observed that the phenotypic change observed not only concerns pigmentation but also energy metabolism. The evolutionary implications of these observations will be discussed

Phenotypic plasticity in anemonefishes: An Eco/Evo/Devo analysis

As poikilotherms, fish exposed to environmental extremes temperatures experience a stressful metabolic challenge, which elicits physiological responses required to maintain cellular homeostasis. These responses include modified glucose or lipid metabolism, altered gene expression and alternative splicing, and endocrine and immune system activity. Additionally, heat seeking may not resolve the homeostatic needs of tropical poikilotherms under unpredictable extreme cold events, which occur frequently due to global climate changes. In spite of the physiological responses that occur in poikilothermic vertebrates, the prevailing notion is that their reduction in metabolic rate is passive. Here, we explored molecular hypothalamic and physiological responses to cold stress in the tropical cichlid, Nile tilapia (Oreochromis niloticus). When exposed to low temperatures, tilapia exhibited complex homeostatic responses, including increased plasma glucose and cortisol concomitant with reduced plasma lactate and metabolic rate. Hypothalamic transcriptome analysis revealed increased oxytocin expression. Blockage of oxytocin signaling using zebrafish CRISPR-knockout lines for oxytocin or its receptors, and pharmacological antagonist in tilapia, further affected temperature-dependent metabolic rate in two cold-exposed tropical fish species. This indicates that oxytocin, a known thermoregulator in homeotherms, actively regulates temperature-related homeostasis in fish. Overall, our findings show that the fish brain actively responds to cold temperature by regulating metabolic physiology. Moreover, we identify oxytocin signaling as an adaptive and evolutionarily conserved metabolic regulator of temperature-related homeostasis. 

Oxytocin centrally regulates temperature-dependent metabolism in fish

Animals respond to environmental challenges with a suite of physiological responses – the stress response. As high temperatures become more frequent and intense globally, determining whether and how individuals vary in their stress response to heat is urgent. While there is an emerging interest in understanding avian stress response to heat, very often, only glucocorticoids are quantified. Here, we tested whether early-life conditions affect multiple traits of nestling stress physiology in response to heat. In the arid-adapted zebra finch, parents emit “heat-calls” when incubating under high temperatures, which adaptively alter offspring growth. We exposed nestlings to prenatal heat-calls or control-calls, and manipulated postnatal nest temperatures. At 13-day old, we collected blood samples in two experiments: under undisturbed “in-nest” conditions, or following a 2.5-hour heat-challenge. As biomarkers of acute, cellular and chronic stress, we measured, respectively, levels of corticosterone (CORT), constitutive heat-shock cognate 70 (HSC70) and stress-inducible heat-shock protein 90 (HSP90), and, the heterophil to lymphocyte (H/L) ratio. We found that none of the stress biomarkers co-varied in either experiment, likely due to distinct time-courses. After the heat-challenge, only the H/L ratio was higher than in the in-nest experiment. Furthermore, in the in-nest experiment, control-call nestlings had a marginally higher H/L ratio than heat-calls nestlings, and both HSPs increased with nest temperatures, in control-call individuals only. These results suggest a higher sensitivity of control-calls nestlings to summer nest temperatures. Overall, our study shows for the first time that a prenatal acoustic signal for heat affects physiological stress responses to postnatal thermal conditions. 

Prenatal acoustic signals and postnatal heat shape nestling stress physiology

Many pesticides are endocrine-active, even if the intended mechanism of action is not specified to directly interfere with outcomes such as development, reproduction, or sex determination. Pyrethroid pesticides, which are commonly used in both North America and the European Union, are based on toxins naturally produced by the chrysanthemum plant, but are chemically enhanced to increase persistence and toxicity to target organisms. These modifications make pyrethroids more likely to interact with endocrine targets such as the nuclear and membrane estrogen receptors, to modify downstream responses like the activity of steroidogenic enzymes, and to ultimately induce organismal responses indicative of endocrine disruption in fish (e.g. changes in gonad size, sex ratio). Previous work from our group confirmed these interactions for specific pyrethroid types, demonstrating that early life exposure can result in impacts that span multiple generations, and that increased temperature can exacerbate these effects. Recent findings indicate that other abiotic factors, such as salinity, can influence the direction and degree of endocrine-related responses, likely due to a combination of osmoregulatory modulation and differential chemical behavior that is dependent upon aqueous salinity. These observations are highly relevant in terms of predicted changes in temperature and salinity regimes over the coming decades, and our findings underscore the importance of understanding responses to endocrine-active compounds in the context of altered environmental conditions.

Endocrine disruption in the context of climate change: understanding responses in fish

On the upper surface of its leafs salvinia molesta has pillar shaped structures which are hydrophilic at the tip and hydrophobic at the shaft. When submerged under water, air is retained between the surface structure. Beside the storage of oxygen this feature enables a variety of further applications. The air layer could serve as barrier to prevent the settlement of biological organisms (antifouling) and reduce wall shear stress if water flows along the surface. These phenomena are from higher interest in case of ships or liquid transport in pipes. If an air retaining structure could be realised on such surfaces, a reduction of frictional drag, fuel consumption and CO2 production could follow. A requirement for this is the understanding of the physics and the influence of fundamental parameters such as structure geometry, size, contact angle of the structure material, pressure, or air saturation of the liquid. In this work the effect of different parameters is presented and discussed using numerical simulation, analytical approaches and experiments.

Air Retaining Surface Structure of Salvinia molesta - Physics, Applications and Challenges1

Muscles perform a number of roles, acting as motor, brakes and shock absorbers. Tendons also perform several roles, acting as transmission and/or elastic springs. But a distinct and perhaps largely overlooked role of isometric muscles and tendons is as functional tension bicycle spokes, becoming loaded in turn through simple geometry, and enabling horizontal translation during vertical weight support without high mechanical power demand. Here, quadrupedal mammal leg structure and function is considered in the context of enabling low mechanical demand at the level of the limb by maintain forces perpendicular to velocity, at the same time as providing low power supply by keeping muscles isometric when loaded, or unloaded when changing in length. These principles provide a simple account for the general structure of the scapula, serratus ventralis and triceps brachii of the forelimb, and the biceps femoris (curiously crossing across the femur), vastus, rectus femoris and tensor fasciae latae of the hindlimb. Different muscles experience tension at different stages in stance, each resulting in linkages where the links are isometric, and the limb supports predominantly vertical forces during horizontal translation.

Legs as linkages: thinking of isometric muscles and tendons as bicycle spokes

Cereal grains are among the most important sources of energy for humans and domestic animals, owing to large and nutritious endosperm tissues. We study nuclear organization and chromatin in seeds of cereals using cultivated barley (Hordeum vulgare subsp. vulgare) - a diploid temperate zone model cereal used for both food and feed. Barley has a large genome (2n = 2x = 14, 1C = 5.1 Gbp) with interphase chromosomes organized in Rabl configuration with centromeres and telomeres clustering at opposite nuclear poles. Though considered to be typical for large cereal genomes, observations of Rabl configuration are based mostly on nuclei from meristematic tissues. We explored interphase chromosome organization in different tissues of developing barley grains and will show that Rabl chromosome organization is not a general rule in barley and it diminishes with the increasing nuclear DNA content and seed age. Furthermore, we performed transcriptome profiling of different barley grain tissues that allowed determining roles of different biological processes and also chromatin regulatory pathways during this critical stage of development. In summary, all these facets of our barley chromatin research create a fruitful environment for future functional analyses of nuclear organization and epigenetic regulation of gene expression in this agriculturally important species.

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Coordination of growth in plants

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SUMOylation regulates plant syntaxin function in innate immunity

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