France

The cellular adaptive response is a defense network that allows cells to survive and proliferate in the presence of stressful factors. These stressors, which include hypoxia, radiation, thermal shock, and heavy metals, damage cells by increasing oxidative stress via increased generation of reactive oxygen species (ROS). The exposure to mild stress (e.g. thermal shock of 40°C) for an extended period can activate the cellular adaptive response and protect the cell against subsequent exposures to lethal stress doses (e.g. lethal thermal shock of 42°C). However, the mechanisms by which this response is activated are still poorly understood. Nrf2, a transcription factor found as a cytosolic heterodimer with Keap1, is activated in response to oxidative stress and translocates to the nucleus where it binds to the antioxidant response element and initiates a host of antioxidant defenses. The role of Nrf2 in the cellular adaptive response is of the utmost importance. Our studies seek to understand 1) ROS generation in response to mild heat stress, 2) how these increased ROS modulate activation of the cellular adaptive response, and 3) the role of Nrf2 in the modulation of ROS generation and activation of the cellular adaptive response. Our results in HeLa cells show that increased ROS at 40°C originate from both mitochondria and NADPH oxidase. Modulation of Nrf2 activity via overexpressor and knockdown cell lines significantly impacts ROS generation during mild heat stress. Elucidating the role of ROS in the establishment of the cellular adaptive response paves the way to new potential therapeutic treatments.

SEB Annual Conference 2022

Oxidative stress induced by mild heat shock at 40°C activates a cell survival response modulated by Nrf2

[![](https://assets.underline.io/uploads/markdown_image/1/image/261902fdf187a88bfa74da226ff77186.png)](https://drive.google.com/file/d/1gUViQJJHCSgM38OhwpDoTdN89evCsZ-7/view?usp=sharing)
[![](https://assets.underline.io/uploads/markdown_image/1/image/1605292c232e6f185c7f87ee38e8502a.png)](https://drive.google.com/file/d/1kPkznVX_6cx-n846z_pUtyVDZql9KImc/view?usp=sharing)


### **Welcome Message**

Welcome to the inaugural **Dutch Lung Congress.** We have all missed our opportunities to network and interact with our colleagues in the last twelve months or more. While our desire was to meet face to face once again, when it became evident that this would not be possible until much later in 2021 (or even into 2022) the hard working committee organizing the Dutch Lung Congress found, what we hope will be, an excellent alternative to facilitate our annual meeting.

The Dutch Lung Congress aims to bring together everyone interested in research related to pulmonary diseases in the Netherlands. We hope this event will provide opportunities for multidisciplinary interactions to enhance our approaches for addressing these challenging diseases. Each session will include input from patients, clinical and basic and/or translational scientists discussing the latest information around the central themes chosen by the organizing committee. One advantage of holding our congress as a virtual event this year is that we will have the opportunity to hear from world leading experts from all over the globe. We hope you enjoy the programme the organizing committee have put together and will engage in dynamic discussions with all the speakers.

One highlight of our congress will be the Young Investigator Session. Remember to join us for this exciting session when we will hear from the presenters who submitted the top scoring abstracts. This promises to be a session full of exciting science that will display the strengths that constitute the future talent in our field.

When we are not listening to these great presentations we can all meet in Gathertown. Within this interactive platform you will be able to meet colleagues and friends for networking. Enjoy a private chat over a virtual coffee, visit the booths of our sponsors and other parties, chat with the Young Investigator Board members or take some timeout in the relaxation spaces. While in Gathertown take the opportunity to visit all the diverse posters on display. Chat live with the presenters or leave them a question on the discussion board for them to engage with you. Make sure you check for any messages each time you enter Gathertown to make the most of your networking opportunities.

We look forward to seeing you during the DLC and the great scientific (and other) discussions we are anticipating during our 2021 meeting.

On behalf of the DLC organizing Committee
 **Janette Burgess
 Chair, DLC Organizing Committee**
![](https://assets.underline.io/uploads/markdown_image/1/image/f461fb275d9667fb2a76a30813388e90.png)



### Special Session: Publieksmiddag
![](https://assets.underline.io/uploads/markdown_image/1/image/77e768dd500e6205175e5cafba59abdc.png)






![](https://assets.underline.io/uploads/markdown_image/1/image/f461fb275d9667fb2a76a30813388e90.png)
**More about the committee team:**:
[Barbro N. Melgert, PhD] (https://drive.google.com/file/d/1q_IIH3JvTivumCUzzsZGX7BgD41HxQmk/view?usp=sharing)
// [Bas Holverda, PhD] (https://drive.google.com/file/d/1QKXHmTTQjgs4OZVuM8doXU055S-fu_uU/view?usp=sharing)
// [Frits Franssen, PhD] (https://drive.google.com/file/d/1d7HRUJaqGuRFzkPN3SVgkbBfqFkDkWq2/view?usp=sharing)
// [Janette Burgess, PhD] (https://drive.google.com/file/d/1VHFlrEN6uCQGeeW3Lj4I8aTw7HsB0GFr/view?usp=sharing)
// [Michel van den Heuvel, PhD] (https://drive.google.com/file/d/1JeOEP8MHxiK_4lONr8UM2HaxdxpkAmA2/view?usp=sharing)
// [Patrick Bhairosing] (https://drive.google.com/file/d/1xI8ORq-g1bbVcrlRDviZ9NhAwanc4dFl/view?usp=sharing)
// [Gimano Amatngalim, PhD] (https://drive.google.com/file/d/1fyVDDKARLNjRk_JZk8_GyhGMjmp6DWHp/view?usp=sharing)
// [Peter Kunst, PhD] (https://drive.google.com/file/d/1QhtB4nQezpAjbfUAcBnINc-Bm3Shy2Lf/view?usp=sharing)
// [Dr. Michel van den Heuvel, Prof., MD, PhD] (https://drive.google.com/file/d/1JBdNEBXZCEikEZhJ6MoD5DhQfXMz3bP6/view?usp=sharing)
![](https://assets.underline.io/uploads/markdown_image/1/image/f461fb275d9667fb2a76a30813388e90.png)

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Dutch Lung Congress

technical paper

Discover the art of creating extraordinary science at SEB’s Annual Conference at Le Corum Conference Center in Montpellier, France from 5 to 8 July 2022.

SEB’s Annual Conference is celebrated for creating the perfect composition of research, new discoveries and collaborative connections. Be creative and innovative by exchanging ideas and knowledge with masters of biology from all over the world.

2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years.

Please register for the conference by clicking on this link:

https://www.sebiology.org/events/seb-annual-conference-2022/registration.html

The site will be open for registered participants closer to the event.

2022 will find over 1500 experimental biologists from across the globe converging on the third-largest French city near the Mediterranean coast and the fastest-growing city in the country over the past 25 years - Montpellier, France

We demonstrate that one-pot consolidated bioprocessing using deep eutectic solvents can improve the extraction of fermentable sugars from the green seaweed, Ulva linza. Ulva biomass was first pretreated with either sulfuric acid, the deep eutectic solvent choline chloride-oxalic acid, or the ionic liquid 1-ethyl-3-methylimidazolium bromide. After pretreatment, we separated solubilised sugars from residual solids. The residual solids were saccharified using green solvent-stable glycosidases that were isolated from a newly identified strain of the ascomycetous fungus, Penicillium oxalicum. The factors affecting this saccharification were quantified from the response surfaces of a Box-Behnken design. Finally, the sugars extracted from Ulva biomass were fermented into bioethanol using a dual yeast culture. We obtained higher bioethanol yields from Ulva biomass with consolidated processing in deep eutectic solvents, compared to lower yields from Ulva biomass with other solvents or two-stage processing.

One-pot processing of Ulva seaweed biomass with deep eutectic solvent-optimized fermentation

The evolution of lignification in vascular plants conferred improved structural support, defence, and water transport, improving the success of plants on land. Despite lignification being a land plant specific expansion, lignin-like compounds have been identified in the cell walls of intertidal seaweeds. Here, I aim to characterise the function and structure of the ‘phycolignin’ fraction of the chlorophyte seaweed Ulva compressa. UV excitation of Ulva cell wall autofluorescence using confocal microscopy induced a blue-green emission profile comparable to that of lignin, which was significantly elevated under hyposaline stress (1.7X fold change to control, ***) and significantly reduced under hypersaline stress (0.5X fold change, ***), indicating a possible osmoprotective role. Cell walls were then isolated and fractionated into pectin, hemicellulose, and ⍺-cellulose to identify this fluorophore, with plant biomass also fractionated as a ‘lignin positive’ control. Several biochemical tests were performed to identify which polysaccharides lignin-like structures were associated with, followed by ATR-FTIR and NMR. ‘Phycolignin’ in Ulva appeared to be associated with cellulose and hemicellulose, with potential aromatic groups identified by ATR-FTIR and 1H NMR (solution-state). Positive identification of lignin within plant extracts showed that Ulva ‘phycolignin’ displayed fundamental structural differences to true lignin. However, the lack of available reference spectra for FTIR of chlorophyte phenolics, and the general insolubility of the Ulva cell wall in conventional NMR solvents limited data interpretation. Better structural characterisation of ‘phycolignin’ in Ulva will increase our understanding on how multicellularity, the metabolome, and stress responses evolved and diverge within the green lineage.

Characterisation of 'phycolignin' in the cell wall of chlorophyte seaweeds

Mitochondria possess a genome, a vestige of their bacterial ancestor. Over the course of evolution, most of the genes of the ancestor have been lost or transferred to the nucleus. In humans, the mtDNA is limited to 37 genes, with little room for evolutionary novelties. This is radically different from bacterial genomes, which are much larger, and in which we can find genes inside other genes. These sequences are called alternatives open reading frames or altORFs, and they have key functions. By ignoring altORFS, have we underestimated the coding potential of the mitogenome? We found a downstream alternative ATG initiation codon in the +3 reading frame of the human mitochondrial nd4 gene. This newly characterized mitochondrial altORF encodes a 99-amino acids long polypeptide termed MTALTND4 with a completely different amino acid sequence from ND4. MTALTND4 is localized in mitochondria and is also found in the plasma, and it impacts mitochondrial physiology. These findings indicate that mitochondrial genes in humans (and other species) have gone unnoticed. This calls into question the evolution of the mitochondrial genome as well as the selection pressures exerted on the mitochondria and the mechanisms allowing the translation of these alternative proteins. We anticipate that more studies on the alternative mitochondrial proteome will expedite the discovery of new mitochondrial genes with key biological roles.

The underestimated protein coding potential of the mitochondrial genome

Birds metabolically remodel their flight muscle prior to migration to meet the high energetic demands of migratory flight. The degree of plasticity mediated by changes in mitochondrial function is poorly understood but could be explained by two non-mutually exclusive hypotheses: variation in mitochondrial quantity or individual mitochondrial function. For each hypothesis, we predicted higher mitochondrial abundance and predict higher substrate oxidative capacity and reduced reactive oxygen species formation during migration, respectively. We evaluated both hypotheses using yellow-rumped warblers (Setophaga coronata) captured during their autumn migration at Long Point, Ontario, Canada. Half of the birds were sampled in the migratory phenotype and the remaining birds were transferred to a short day photoperiod (9L:15D) and sampled in a non-migratory phenotype. We measured mitochondrial abundance via citrate synthase maximal activity in the pectoralis major flight muscle. We investigated mitochondrial function via high-resolution fluororespirometry on isolated pectoralis mitochondria. We measured O2 consumption and H2O2 formation rates from oxidation of lipid and carbohydrate substrates. We also measured oxygen consumption specific to electron transport system protein complexes using complex-specific substrates and inhibitors. Citrate synthase activity and mitochondrial respiration for each substrate and complexes II, III and IV was higher in the migratory phenotype, yet H2O2 formation rates were similar between phenotypes. These data suggest that pectoralis mitochondrial abundance and oxidative capacity are elevated during migration without increasing reactive oxygen species formation. Our findings support both hypotheses and indicate that mitochondrial function mediates seasonal metabolic plasticity in the pectoralis flight muscle of migratory songbirds.

Flight muscle mitochondria are seasonally plastic in a migratory songbird

Mandibular kinetics and associated occlusal relations have been a significant focus of physiological studies of mammalian mastication. However, soft tissue deformations of the masticatory apparatus are essential to mastication. Whereas the jaw muscles, ligaments and temporomandibular joint (TMJ) articular disc are vital for jaw movements, perioral muscles and the tongue are key to bolus management and transport. We synthesize deformations of these soft tissue structures as the jaw moves during chewing and propose future avenues of research investigating soft tissue dynamics during mastication. Jaw muscles shorten 5-10% and cheek muscles over 15% during mastication, and their bulging creates pressure on adjacent bones and ligaments. Muscle contraction moves and loads the TMJ, causing the disc and capsule to elongate anteroposteriorly during jaw closing. During jaw opening, muscles lengthen, stretching aponeuroses 4-5%. Chewing side, food hardness, regional and individual differences all modify these effects. The most important soft tissue for mammalian mastication is, however, the tongue, and it undergoes the largest deformations. There are differences in how various species use the tongue during mastication, but all species examined exhibit regional complexity in the amplitude and timing relative to jaw movements. In pigs, for example, the anterior tongue lengthens as the jaw closes, reaching maximum length around minimum gape, then quickly shortens as jaw opening begins. Posterior regions lengthen during jaw closing and opening but shorten much later during opening. Synthesizing these functional roles emphasizes the significance of soft tissue mechanics in masticatory performance.

No Bones About It: Soft Tissue Dynamics during Mammalian Mastication

Jaw movements during mammalian chewing drive occlusion and food breakdown. As important as jaw movements are for chewing, soft tissues within the oral cavity are essential for handling the food bolus throughout the chewing sequence. For instance, tongue movements and deformations (1) position the food between the tooth rows, (2) help mix the bolus with saliva to initiate digestion and improve swallowability, and (3) transport the bolus to the oropharynx to initiate the swallow. Tongue movements and deformations must be coordinated with jaw movements to ensure chewing performance and efficiency as well as to avoid self-injury, and previous research shows that disruption of the sensory input from the tongue alters jaw movements during chewing in pigs. Even though jaw and tongue movements during mammalian chewing are investigated extensively, their coordination pattern(s) have received less attention. We present three different approaches to quantify the correlation between jaw movements (opening and closing, mediolateral deviation) and tongue movements (positional changes such as protraction-retraction) and deformation (length and width changes) during chewing in pigs. At the sequence level, cross-correlations are used to calculate the lag in time between tongue and jaw kinematics. Cross-correlation analyses can also be carried out at the cycle level, albeit with less statistical power. Finally, functional data analysis standardizes and resamples the data to test for jaw-tongue correlation at each time point, essentially testing bivariate correlation continuously throughout the chewing cycle. We discuss the benefits and limitations of each methodology to emphasize the impact of combining analytical tools.

Jaw-tongue coordination during chewing in Pigs

Vertebrate feeding is a complex process with multiple aspects of integration, including among anatomical systems and physiologic levels. Success in feeding, both in enhanced performance and avoidance of failure, is a mission-critical function for all animals. Hiiemae and Crompton (1985) argued for this integration as the basis of understanding vertebrate feeding in general, and mammalian feeding in specific. The return to this perspective within the comparative biology/ biomechanics/ evolution community is reflected in the enthusiasm for this symposium . 

Feeding—from ingestion through mechanical and chemical processing to transport and the ultimate swallow into the mid-GI system—is a continuous process through what was embryologically and evolutionarily a simple tube. Irrespective of anatomical and evolutionary elaboration, this basic tube is the foundation of the function and survival of the organism. Focusing on specific system functions (prey capture or mastication) provides insight into how natural selection may act on regional anatomy. Yet, consideration of the entire gastrointestinal tract and its outgrowths is necessary to understand how natural selection operates on the feeding performance of the organism as a whole. 

Neurophysiologic integration produces the function or behaviour of feeding. Performance in feeding is ultimately a feedback loop, taking sensory input from soft tissues and bones and converting that information into muscular activation in the central nervous system (CNS), including the brain stem and the cerebral cortex. Focusing on individual parts or specific levels of this elaborate system has provided an essential detail of how the system works. Integration among these individual functional components as well as consideration of downstream gastrointestinal structure and function may yield further insights into fundamental constraints and key evolutionary pressures on this essential system. Mammalian feeding is an exemplar of how both anatomical and physiologic integration have provided both the basis for the variation in mammalian evolution as well as the limits on what is possible. 


Hiiemae KM, Crompton AW. Mastication, food transport and swallowing. In: Hildebrand M, Bramble D, Liem K, Wake D, editors. Functional Vertebrate Morphology. Cambridge: Harvard University Press; 1985. pp. 262–90.








Integration across anatomy and physiology is the basis for understanding feeding behaviour in vertebrates

Elasmobranch fishes (sharks and rays) display a skin covered in hypermineralized scales that resemble teeth. These structures are continuously regenerated over the individual’s lifetime, they display a range of morphological variations within one individual, but also between species. Scales and skin are under a wide set of selective pressures, including general resistance to parasites, female protection against male biting during mating, or hydrodynamism in pelagic species. 
The development and regeneration of such structures raises several issues that can be addressed by developmental biologists, such as:  How is the organisation of these arrays of scales regulated? How can the diversity in shape be generated within one individual? How are other physiological systems (e.g., the sensory system) organised over these locally mineralized surfaces? Here I will discuss the contribution of recent studies on the lesser spotted catshark Scyliorhinus canicula to highlight several developmental aspects related to these issues, integrating morphological descriptions, development modelling and transcriptomic data. 

Skeleton in the skin? Teeth in the skin!

The rate at which animals acquire and use energy is an important factor that affects many aspects of animal performance, including reproductive output and thermoregulatory capabilities. Therefore, understanding the factors limiting energy budgets has remained one of the central issues in ecological and evolutionary physiology. We used a unique experimental evolution model – lines of bank voles (Myodes glareolus) selected for high rate of swim-induced aerobic metabolism (VO2swim, A-lines), which evolved a 70% higher VO2swim than that observed in unselected control (C) lines – and asked how the BMR, reproductive output, and thermoregulatory capabilities evolved in response to the selection. After 25 generations, animals from the A-lines achieve over 30% higher forced running maximum metabolic rate than those from unselected, control (C) lines. The selection has also increased the forced running maximum metabolic rate by about 30%37. Voles from the A-lines spend more time on active swimming, and, unlike the C-line voles, during the swimming trial work up to their physiological performance limit they achieve during running. In these lines the animals have developed an increased metabolic capacity, and we tried to understand the mechanism behind it. 




The physiological effects of experimental evolution of aerobic exercise performance in bank voles

Life-history variation emerges due to trade-offs in energy allocation, suggesting energetics shapes life-history evolution. The Atlantic salmon (Salmo salar) exhibits high diversity in the age-at-maturity trait, which is largely controlled by two genomic regions, vgll3 and six6.  This simple genetic control of age-at-maturity enables implementing genomic prediction methods to test potential genetic correlations, and subsequent co-evolution, between age-at-maturity and physiological traits. Here, we tested how variation in these life-history genomic regions affect the central metabolic phenotypes: standard and maximum metabolic rates, and aerobic scope in juvenile Atlantic salmon. We found no evidence for covariation between standard metabolic rate and the two life-history loci. However, we found that one of the life-history loci affected the maximum metabolic rate and aerobic scope, and that the two loci showed physiological epistasis in the control of maximum metabolic rate. We then measured proxies of aerobic and anaerobic capacities from four tissues to study how tissue-specific energetics can mediate genetic variation in life-history.  The results help us to better understand the mechanistic basis of life-history variation as well as metabolic constraints on life-history evolution.

Oxidative stress induced by mild heat shock at 40°C activates a cell survival response modulated by Nrf2

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