Museum specimen digitization is a rapidly growing practice, with considerable potential for cataloguing, research, and public access to museum collections. The Hong Kong Biodiversity Museum (HKBM) boasts Hong Kong’s largest natural history collection, >55,000 specimens across the taxonomic spectrum, from plants to insects to vertebrates. However, constraints to space, funding, and manpower, pose challenges to sharing our resources. In a joint project, we are building a digital collection to expand collection accessibility and create new avenues for public interaction and anatomy education, using a diversity of approaches for interacting with 3D data. We have produced more than 30 CT/microCT scans of dry specimens, primarily skulls, and utilized open-source software (e.g. 3D Slicer, Blender) to create 3D prints for hands-on experience. From our data, for example, we generate up-scaled 3D-printed models to interactively teach CityU anatomy students cranial nerve pathways. Partnering with local start-up OAO and Scan the World (STW), we use their scanning and visualization platforms (e.g. OAO’s GIMII app) to generate and annotate models of taxidermy, skeletal and wet dissected specimens. GIMII’s AR capabilities and STW’s 3D engine give museum guests and students opportunities to explore items that cannot be easily physically displayed (e.g. the museum’s largest specimens), with STW’s browser-based platform allowing integrated annotation, commenting, and LLM-powered interaction to support multi-user, fully navigable, 3D-scanned environments with drag-and-drop model placement. We discuss how digitization is allowing the museum to modernize, reach a broader audience and transcend limitations, outlining expansion of resources in classes and museum exhibitions.