Background Long-duration spaceflight exposes astronauts to immense physical and psychological stress, potentially impairing neurocircuitry and motor coordination. Recent literature has revealed decreased density in several white matter tracts associated with motor control and coordination alongside well-established deficits in coordinative function post-spaceflight. Virtual reality (VR) could potentially be used to monitor and enhance neurocognitive resilience in space environments. This project involved conducting a literature review, creating a study design, and submitting a NASA grant to fund a VR training program that will be implemented during a future space mission in October 2025. Methods We have designed and implemented a VR training program with several games capturing pupillometry, eye tracking, and body kinematic data to test various aspects of cognitive control and psychomotor function. Performance data will be collected by the REACT Neuro VR System through biometric sensors and task analytics. Evaluation will involve outcome measures including ocular dynamics, oculo-motor coordination, accuracy, and reaction time, to compare baseline assessments with post-spaceflight outcomes. Overall task performance will be based on the number of difficulty levels successfully passed. Additionally, astronauts will undergo specialized MRI imaging to quantify white matter changes and associate them with VR metrics. Results Preliminary activities include literature synthesis and grant preparation, building a robust framework for this project. Anticipated results, as demonstrated in a test cohort of Air Force C-17 pilots earlier this year, include detection of early psychomotor decline after high-altitude flight and new evidence for the use of VR training to preserve motor coordination and strength in the astronaut population. Conclusion This VR analysis program provides a new method for neurocognitive monitoring during spaceflight. It may also support the role of neuroplasticity in psychomotor changes observed after spaceflight. Limitations include small participant sample sizes and delays between spaceflight and MRI imaging, while future directions involve scaling this program to larger cohorts and integrating more neurofeedback metrics. After demonstrating REACT Neuro’s potential to track and rehabilitate coordinative and cognitive deficits in astronauts, we see incredible potential for translating these techniques to patients with similar ailments in the general population.