Challenges and Innovative Strategies of Identifying Individual Probiotic Species in MultiSpecies Formulations: A Finished Product Manufacturer’s In-House QC Lab Approach

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2025 AOAC INTERNATIONAL Annual Meeting & Exposition

•

August 27, 2025

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San Diego, United States

Biodiversity and symbiosis are the prevailing themes that have helped expand the popularity of multispecies probiotic formulations. Research endeavors on the topic have demonstrated that probiotic blends provide more symbiotic and beneficial effects, particularly on gut health. Currently, there is an increasing demand from customers to deliver at least 10 probiotic species per dosage. This posed a challenge to probiotic formulators, finished product manufacturers, and their quality control laboratories. Formulation and manufacturing should be intricately designed to ensure that every production process conserves all the probiotic species up to their final form (e.g., capsules or tablets). Ultimately, the Quality Control Laboratory will then have to verify the identity of each probiotic species as promised for each dosage. Most probiotic product manufacturers contract with expert laboratories for multispecies identification testing. This incurs a substantial cost, not only in the testing costs but also in the turnaround time of the test. The average price of testing for the identification of 10 different species of probiotics ranges from $1,000 to $ 3,000 per sample, with the fastest turnaround time being 3 days. The challenge of verifying product purity is a strong motivator for the in-house laboratory to explore innovative strategies to reduce the cost of identification testing and turnaround time. The speaker will present new developments in probiotic identification testing that aim to bring multi-species probiotic identification testing in-house, lowering the cost of identifying multispecies probiotics to less than $500 per sample and achieving a turnaround time of 1 hour.

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Next from 2025 AOAC INTERNATIONAL Annual Meeting & Exposition

Overcoming Obstacles: Technologies for the Enumeration and Identification of Probiotic Multi-Strain Blends

Overcoming Obstacles: Technologies for the Enumeration and Identification of Probiotic Multi-Strain Blends

2025 AOAC INTERNATIONAL Annual Meeting & Exposition

Andrzej Benkowski
Andrzej Benkowski

27 August 2025

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