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keywords:
biology cell microscopy
cv
Reconstructing the intricate local morphology of neurons as well as their long-range projecting axons can address many connectivity related questions in neuroscience. While whole-brain imaging at single neuron resolution has recently become available with advances in light microscopy, segmenting multiple entangled neuronal arbors remains a challenging instance segmentation problem. Split and merge mistakes in automated tracings of neuronal branches can produce qualitatively different results and represent a bottleneck of reconstruction pipelines. Here, by extending the notion of simple points from digital topology to connected sets of voxels (i.e. supervoxels), we develop a topology-aware neural network based segmentation method with minimal overhead. We demonstrate the merit of our approach on a newly established public dataset that contains 3-d images of the mouse brain where multiple fluorescing neurons are visible as well as several other 2-d segmentation datasets.