Background Simulation training is becoming increasingly recognized as a powerful learning tool in medical education, as it offers a unique advantage to practice surgical skills in a low-risk environment. The Ophthalmology Interest Group at the Long School of Medicine, in partnership with the UT Health Ophthalmology Department, created an Eye Suture Workshop for medical students. The aim of this workshop was to provide students with hands-on experience and exposure to the field of ophthalmology, as well as to assess knowledge gained in a prospective cohort study. Methods Twelve first year medical students participated in an eye suture workshop. The students were divided into six groups of two and given cow eyes for suturing under the supervision of an ophthalmology faculty member and residents. Pre- and post-workshop surveys using 1-10 Likert scale questions were administered to participants to assess the degree of agreement with questions on surgical and ophthalmology interest, comfort with microscope use, and confidence in suturing skills. Suture technique knowledge and ocular anatomy were also assessed through multiple choice and matching quizzes. Results were analyzed in STATA using a Student’s T-Test and Wilcoxon Signed Rank Test (p <0.05 indicating significance). Results Self-reported interest in both a surgical specialty (p-value = 0.0455) and in ophthalmology (p-value = 0.0462) increased significantly following the workshop, as well as student confidence in suture skills (p-value = 0.002) and microscope comfort (p-value = 0.0016). Basic suturing technique skills (p-value = 0.0001) and ocular anatomy (p-value = 0.0462) similarly improved following the workshop. On additional post-test Likert questions that assessed agreement with statements about their perception of the workshop, students rated that receiving hands-on demonstrations of eye suturing skills was helpful (mean: 9.83 (SD: 0.39)) and that they would recommend this workshop to other preclinical students (mean: 9.83 (SD: 0.39)). Conclusion This workshop proved to be a highly effective educational intervention by improving student confidence in suturing techniques and increasing knowledge of suture skills and ocular anatomy, as well as increasing interest in ophthalmology and in surgical specialties. Limitations to this study include a lack of distant outcome measurements to assess persistence of the knowledge gained. Furthermore, the small sample size due to capacity limits in the lab reduces the power of this study.