Objective: The purpose of this study is to investigate the connection between the prevalence of metabolic syndrome and circadian misalignment in night shift workers. Understanding the impact of circadian rhythm disturbance on metabolic health is essential, especially in light of the growing incidence of night shift employment and its possible health consequences. Methods: We searched Pubmed, Google Scholar, Cochrane Library and PLOS Medicine using relevant keywords such as “circadian misalignment, circadian rhythm disorders, circadian rhythm, metabolic syndrome, night-shift workers, insulin resistance”. We used “AND” and “OR” boolean operators for these keywords. We included peer-reviewed studies in humans that were published in the last 10 years (i.e., 2014–2024). The reference list of selected articles was also screened for relevant studies. Outcomes of interest were cardiometabolic outcomes, including obesity/body mass index (BMI), cardiovascular disease, diabetes, high blood pressure, fatty liver disease, and insulin resistance. This review focused on clinical trials, randomized clinical trials and meta-analysis. However, relevant observational studies were also included. There were no limitations on sample size or study location; however, only studies published in English and with adult participants over the age of 19 were considered in this review. Results: A total of 37 articles met inclusion criteria and were added to the review. The key findings from these results show that compared to day workers, night shift workers have a higher chance of acquiring metabolic syndrome. Among night shift workers who experienced circadian misalignment, key metabolic syndrome components such as obesity, insulin resistance, dyslipidemia, and hypertension were common. The suggested pathophysiological processes include interrupted sleep patterns, modified eating behaviors, hormone abnormalities, and impaired glucose metabolism. Conclusion: This review provides significant evidence that circadian misalignment in night shift workers contributes to the development of metabolic syndrome. The disruption of natural sleep-wake cycles contributes to metabolic syndrome, emphasizing the importance of tailored therapies to reduce its effects. Interventions targeting the reduction of circadian disturbance, such as the implementation of regulated lighting conditions, optimizing work schedules, and lifestyle adjustments, are crucial for minimizing the metabolic health hazards linked to night shift work. Additionally, implementing interventions that promote circadian alignment, such as improving sleep hygiene, and educating people about the benefits of regular sleep patterns, may help minimize the incidence of metabolic syndrome in this sensitive population. Future research should prioritize conducting longitudinal studies and intervention trials in order to demonstrate a causal relationship and develop efficacious preventive interventions. Keywords: circadian misalignment, circadian rhythm disorders, circadian rhythm, night shift workers, insulin resistance, cardiovascular diseases, metabolic syndrome.