While progress has been made in understanding the neurodegenerative mechanisms that lead to cell death in Parkinson's disease (PD), early causal pathogenic events are not clear. Converging findings point at endolysosomal (EL) system dysfunction as the early mechanism and key pathway affected in PD. However, the exact mechanism by which alpha-synuclein (aSyn) aggregates, also called Lewy Bodies (LBs), disrupt the EL system remain elusive. To answer this question, our group created a new optogenetic-based model of PD that allows for the real-time induction of aSyn aggregates under the blue light control, mimicking all cardinal LBs features. This Light-Inducible Protein Aggregation (LIPA) system allows us to explore unsolved questions related to early interactions between the EL system, LBs and PD pathogenesis. Using the LIPA inside living cells, we were able to study the direct impact of our aggregates on vesicle homeostasis by investigating the interactions between our LIPA aggregates and the EL system using the super-resolution microscopy STED in combination with transmission electron microscopy (TEM). STED microscopy offered us a better understanding of the interactions between LBs and trafficking vesicles showing early interactions with vesicles such as the early endosomes (EEA-1) but also the degradation vesicles (LAMP1/2A) characterized by multiple co-localization with those markers. Interestingly, those vesicles were differentially interacting with aSyn aggregates overtime. The TEM realized on our purified aggregates, revealed that they are composed of multiple vesicles and shown distorted organelles. Those results allowed us to better understand how aSyn aggregates impact the trafficking of the vesicles inside the cell overtime.

Unveiling the role of the endolysosomal system in the pathogenesis of Parkinson's disease

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PS-1-C -  Disorders of the Nervous System

poster

Colleagues and Friends:

I am very happy to welcome you to the 14th annual Canadian Neuroscience Meeting, and to the first virtual edition of our meeting. I want to highlight the important work the CAN meeting chair, **Roger Thompson** and co-chair, **Alyson Fournier**, did to put together the great program we are offering this year. Moving to a virtual format required a complete re-design of the program, and both Roger and Alyson did a splendid job in providing us with an appealing and well adapted program for our first virtual event. I wish to thank them for this very important effort.

Highlights of this year’s scientific meeting include invited speakers **Michael Hausser, Franck Polleux, Gwyneth Card, Magdalena Götz**and Brain Prize winner **Huda Zoghbi**. Plenary and parallel symposia, proposed by our members complete our diverse [scientific program.](https://can-acn.org/meeting-2021/program-2021/)

The 2021 Advocacy session, chaired and organized by CAN advocacy committee chair **Karun Singh**, will feature a special lecture and question period with Member of Parliament and past Science Minister **Kirsty Duncan**. This will be followed by a short overview of CAN’s advocacy activities over the last year. We invite you to participate in this session for insight from Dr. Duncan on the workings of Parliament Hill, and to learn more about what CAN has been doing to promote neuroscience research in Canada.

We are also excited to host **Lisa Willis** in the Equity Diversity and Inclusion session, organized by EDI committee chair **Jibran Khokhar**.

A special highlight of our meeting will be the lectures by award-winning young neuroscientists. We are please to host the CAN 2020 Young Investigator Award winner **Mihaela Iordanova**, and CAN 2021 Young Investigator Award winners **Aaron Phillips** and **Tabrez Siddiqui**. Highlights will also include presentation by the top three winners of the CAN-CIHR-INMHA Brain Star Award winners, **Claire Gizowski, Nuria Daviu Abant** and **Lukas Tamayo-Orrego**.

Independently organized satellite symposia complement and complete this exciting program, check them out here: https://can-acn.org/meeting-2021/2021-satellite-symposia/

Some of the most engaging sessions are always the poster sessions featuring the work of our trainees, so make sure to log in to Gather Town during the poster sessions to chat with the presenters.
We hope you enjoy this program, and look forward to welcoming you, virtually.

Shernaz Bamji

President of the Canadian Association for Neuroscience

![](https://assets.underline.io/uploads/markdown_image/1/image/b9aea25343a87d5aac3b9367dc780974.jpg)



Morgan Bérard

1

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Unveiling the role of the endolysosomal system in the pathogenesis of Parkinson's disease

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